Ketogenic diet induces p53-dependent cellular senescence in multiple organs.

A ketogenic diet (KD) is a high-fat, low-carbohydrate diet that leads to the generation of ketones. While KDs improve certain health conditions and are popular for weight loss, detrimental effects have also been reported. Here, we show mice on two different KDs and, at different ages, induce cellular senescence in multiple organs, including the heart and kidney.

Mitochondrial ACSS1-K635 acetylation knock-in mice exhibit altered metabolism, cell senescence, and nonalcoholic fatty liver disease.

Acetyl-CoA synthetase short-chain family member 1 (ACSS1) uses acetate to generate mitochondrial acetyl-CoA and is regulated by deacetylation by sirtuin 3. We generated an ACSS1-acetylation (Ac) mimic mouse, where lysine-635 was mutated to glutamine (K635Q). Male mice were smaller with higher metabolic rate and blood acetate and decreased liver/serum ATP and lactate levels.

NMR Study of CO Capture by Butylamine and Oligopeptide KDDE in Aqueous Solution: Capture Efficiency and Gibbs Free Energy of the Capture Reaction as a Function of pH.

We have been interested in the development of rubisco-based biomimetic systems for reversible CO capture from air. Our design of the chemical CO capture and release (CCR) system is informed by the understanding of the binding of the activator CO ( CO ) in rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase). The active site consists of the tetrapeptide sequence Lys-Asp-Asp-Glu (or KDDE) and the Lys sidechain amine is responsible for the CO capture reaction.

Manganese Superoxide Dismutase Acetylation and Regulation of Protein Structure in Breast Cancer Biology and Therapy.

The loss and/or dysregulation of several cellular and mitochondrial antioxidants' expression or enzymatic activity, which leads to the aberrant physiological function of these proteins, has been shown to result in oxidative damage to cellular macromolecules. In this regard, it has been surmised that the disruption of mitochondrial networks responsible for maintaining normal metabolism is an established hallmark of cancer and a novel mechanism of therapy resistance. This altered metabolism leads to aberrant accumulation of reactive oxygen species (ROS), which, under specific physiological conditions, leads to a potential tumor-permissive cellular environment.

Computational Investigation of the Thermochemistry of the CO Capture Reaction by Ethylamine, Propylamine, and Butylamine in Aqueous Solution Considering the Full Conformational Space via Boltzmann Statistics.

Rubisco is the enzyme responsible for CO fixation in nature, and it is activated by CO addition to the amine group of its lysine 201 side chain. We are designing rubisco-based biomimetic systems for reversible CO capture from ambient air. The oligopeptide biomimetic capture systems are employed in aqueous solution.

Video colorimetry of single-chromophore systems based on vector analysis in the 3D color space: Unexpected hysteresis loops in oscillating chemical reactions.

Colorimetry, the quantitative determination of color, usually of a digital image, has useful applications in diverse areas of research. Many methods have been proposed for translating the RGB data of an image to obtain concentration information. Among the many methods for RGB analysis, we focus on the vector projection method (VP), which is based on a vector analysis in 3D RGB color space.