Synthetic biology approaches for enhancing safety and specificity of CAR-T cell therapies for solid cancers.

CAR-T cell therapies have been successful in treating numerous hematologic malignancies as the T cell can be engineered to target a specific antigen associated with the disease. However, translating CAR-T cell therapies for solid cancers is proving more challenging due to the lack of truly tumor-associated antigens and the high risk of off-target toxicities. To combat this, numerous synthetic biology mechanisms are being incorporated to create safer and more specific CAR-T cells that can be spatiotemporally controlled with increased precision.

Physical and Biological Characterization of the Gamma-Irradiated Human Cornea.

Purpose: To compare the physical and biological characteristics of commercial gamma-irradiated corneas with those of fresh human corneas and to determine suitability for transplantation.

Methods: The physical properties of gamma-irradiated and fresh corneas were evaluated with respect to light transmittance, hydration (swelling ratio), elastic modulus (compressive modulus by the indentation method), matrix organization (differential scanning calorimetry), and morphology (light and transmission electron microscopy). The biological properties of the gamma-irradiated cornea, including residual cell content and cellular biocompatibility, were evaluated by quantifying DNA content and measuring the proliferation rate of human corneal epithelial cells, respectively.

Paraxial full-field cloaking.

We complete the 'paraxial' (small-angle) ray optics cloaking formalism presented previously [Opt. Express 22, 29465 (2014)], by extending it to the full-field of light. Omnidirectionality is then the only relaxed parameter of what may be considered an ideal, broadband, field cloak.

Paraxial ray optics cloaking.

Despite much interest and progress in optical spatial cloaking, a three-dimensional (3D), transmitting, continuously multidirectional cloak in the visible regime has not yet been demonstrated. Here we experimentally demonstrate such a cloak using ray optics, albeit with some edge effects. Our device requires no new materials, uses isotropic off-the-shelf optics, scales easily to cloak arbitrarily large objects, and is as broadband as the choice of optical material, all of which have been challenges for current cloaking schemes.

Application of a collagen-based membrane and chondroitin sulfate-based hydrogel adhesive for the potential repair of severe ocular surface injuries.

This study was performed to evaluate the potential of a chondroitin sulfate-polyethylene glycol (CS-PEG) adhesive and collagen-based membrane (collagen vitrigel, CV) combination as a method to treat penetrating ocular injuries on the battlefield and to improve this method with two technologies: an antibiotic releasing CS-PEG adhesive and a corneal shaped CV. Burst testing using porcine cadaveric eyes, high-performance liquid chromatography, the Kirby-Bauer bacterial inhibition test, and CV implantations on the live and cadaveric rabbit eyes were performed. The ocular burst test showed CS-PEG adhesive could successfully repair 5-mm to 6-mm length wounds in the corneal and corneoscleral regions but would require CS-PEG + CV to treat larger wounds similar to those seen on the battlefield.

Amplitude-only, passive, broadband, optical spatial cloaking of very large objects.

We demonstrate three amplitude cloaks that can hide very large spatial objects over the entire visible spectrum using only passive, off-the-shelf optics. The cloaked region for all of the devices exceeds 10⁶ mm³, with the largest exceeding 10⁸ mm³. Although unidirectional, these cloaks can hide the cloaked object, even if the object is transversely illuminated or self-illuminated.