Remote Measurements of Tear Electrolyte Concentrations on Both Sides of an Inserted Contact Lens.

In this paper, a method is described to perform ion concentration measurements on both sides of an inserted contact lens, without physical contact with the eye or the contact lens. The outer surface of an eye is covered with a tear film that has multiple layers. The central aqueous layer contains electrolytes and proteins.

Plasmon- and Waveguide-Coupled Fluorescence at the Ultraviolet Region.

Surface plasmon-coupled emission (SPCE) has been well studied for its coupled, directional, and enhanced P-polarized radiation due to the interactions of fluorophores with surface plasmon polaritons (SPPs) on thin metal films. Such surface plasmon polariton-assisted directional fluorescence has various applications in biosensing. Herein, we demonstrate 2-aminopurine (2AP, a UV-absorbing and -emitting fluorophore) emission coupling to modes in aluminum-based plasmon-coupled waveguides (Al-PCWs).

TLR5-Derived, TIR-Interacting Decoy Peptides to Inhibit TLR Signaling.

TLR5, which is activated by flagellin, plays an important role in initiating immune response to a broad spectrum of motile bacterial pathogens. TLRs induce intracellular signaling via dimerization of their TIR domains followed by adapter recruitment through multiple interactions of receptor and adapter TIRs. Here, a library of cell-permeable decoy peptides derived from the TLR5 TIR was screened for TLR5 signaling inhibition in the HEK-Blue-mTLR5 reporter cell line.

Far-field optical imaging of surface plasmons with a subdiffraction limited separation.

When an ultrathin silver nanowire with a diameter less than 100 nm is placed on a photonic band gap structure, surface plasmons can be excited and propagate along two side-walls of the silver nanowire. Although the diameter of the silver nanowire is far below the diffraction limit, two bright lines can be clearly observed at the image plane by a standard wide-field optical microscope. Simulations suggest that the two bright lines in the far-field are caused by the unique phase distribution of plasmons on the two side-walls of the silver nanowire.

Planar photonic chips with tailored angular transmission for high-contrast-imaging devices.

A limitation of standard brightfield microscopy is its low contrast images, especially for thin specimens of weak absorption, and biological species with refractive indices very close in value to that of their surroundings. We demonstrate, using a planar photonic chip with tailored angular transmission as the sample substrate, a standard brightfield microscopy can provide both darkfield and total internal reflection (TIR) microscopy images with one experimental configuration. The image contrast is enhanced without altering the specimens and the microscope configurations.

Sulforaphane covalently interacts with the transglutaminase 2 cancer maintenance protein to alter its structure and suppress its activity.

Type 2 transglutaminase (TG2) functions as an important cancer cell survival protein in a range of cancers including epidermal squamous cell carcinoma. TG2 exists in open and closed conformations each of which has a distinct and mutually exclusive activity. The closed conformation has GTP-binding/GTPase activity while the open conformation functions as a transamidase to catalyze protein-protein crosslinking.

Converting the guided-modes of Bloch surface waves with surface pattern.

The guided-modes of Bloch surface waves, such as the transverse electric modes (TE00 and TE01 modes), can simultaneously exist in a low-refractive-index ridge waveguide with subwavelength thickness that are deposited on an all dielectric one-dimension photonic crystal. By using the finite difference frequency domain method, coupled mode theory and finite-difference time-domain method, the conversion between the guided-modes has been investigated. This conversion can be realized in a broadband wavelength with surface pattern of this low-index ridge.

Fluorophore Coupling to Internal Modes of Bragg Gratings.

Multilayer structures with two dielectrics having different optical constants and no structural features in the - plane can display photonic band gaps (PBGs) and are called one-dimensional photonic crystals (1DPCs). If the top layer thickness is carefully selected, the electromagnetic energy can be trapped at the top surface. These highly enhanced fields are called Bloch surface waves (BSWs).

Fluorescence Coupling to Internal Modes of 1D Photonic Crystals Characterized by Back Focal Plane Imaging.

The coupling of fluorescence with surface electromagnetic modes, such as surface plasmons on thin metal films or Bloch surface waves (BSW) on truncated one-dimensional photonic crystals (1DPC), are presently utilized for many fluorescence-based applications. In addition to the surface wave, 1DPCs also support other electromagnetic modes that are confined within the 1DPC structure. These internal modes (IMs) have not received much attention for fluorescence coupling due to lack of spatial overlap of their electric fields with the surface bound fluorophores.

Vortex Beam Generation by Spin-Orbit Interaction with Bloch Surface Waves.

Axis-symmetric grooves milled in metallic slabs have been demonstrated to promote the transfer of Orbital Angular Momentum (OAM) from far- to near-field and vice versa, thanks to spin-orbit coupling effects involving Surface Plasmons (SP). However, the high absorption losses and the polarization constraints, which are intrinsic in plasmonic structures, limit their effectiveness for applications in the visible spectrum, particularly if emitters located in close proximity to the metallic surface are concerned. Here, an alternative mechanism for vortex beam generation is presented, wherein a free-space radiation possessing OAM is obtained by diffraction of Bloch Surface Waves (BSWs) on a dielectric multilayer.

Sodium-Sensitive Contact Lens for Diagnostics of Ocular Pathologies.

The ability to measure all the electrolyte concentrations in tears would be valuable in ophthalmology for research and diagnosis of dry eye disease (DED) and other ocular pathologies. However, tear samples are difficult to collect and analyze because the total volume is small and the chemical composition changes rapidly. Measurements of electrolytes in tears is challenging because typical clinical assays for proteins and other biomarkers cannot be used to detect ion concentrations tears.

Fluorescent contact lens for continuous non-invasive measurements of sodium and chloride ion concentrations in tears.

Rapid and non-invasive measurement of hydration status is medically important because even mild levels of dehydration can have a significant impact on physical and cognitive performance. Despite the potential value of determining whole-body hydration based on the electrolytes found in tears, very few tests are available. An area of intense interest is the development of a contact lens which could measure ion concentrations in tears, specifically that of sodium (Na) and chloride (Cl) ions, the dominant electrolytes in blood plasma and tears.

Optical Trapping with Focused Surface Waves.

Near-field optical trapping can be realized with focused evanescent waves that are excited at the water-glass interface due to the total internal reflection, or with focused plasmonic waves excited on the water-gold interface. Herein, the performance of these two kinds of near-field optical trapping techniques is compared using the same optical microscope configuration. Experimental results show that only a single-micron polystyrene bead can be trapped by the focused evanescent waves, whereas many beads are simultaneously attracted to the center of the excited region by focused plasmonic waves.

Trapping metallic particles using focused Bloch surface waves.

Metallic particles are promising for applications in various areas, including optical sensing, imaging and electric field enhancement-induced optical and thermal effects. The ability to trap or transport these particles stably will be important in these applications. However, while traditional optical tweezers can trap metallic Rayleigh particles easily, it is difficult to trap metallic mesoscopic/Mie particles because of the strong scattering forces that come from the far-field trapping laser beam.

Switchable Assembly and Guidance of Colloidal Particles on an All-Dielectric One-Dimensional Photonic Crystal.

Dielectric multilayer photonic-band-gap structures, called one-dimensional photonic crystals (1DPCs), have drawn considerable attention in the fields of physics, chemistry, and biophotonics. Here, experimental results verify the feasibility of a 1DPC working as a substrate for switchable manipulations of colloidal microparticles. The optically induced thermal convective force on a 1DPC can assemble colloidal particles that are dispersed in a water solution, while the photonic scattering force on the same 1DPC caused by propagating evanescent waves can guide these particles.

Coupling of Fluorophores in Single Nanoapertures to Tamm Plasmon Structures.

Metal nanostructures (such as plasmonic antennas) have been widely demonstrated to be excellent devices for beaming and sorting the fluorescence emission. These effects rely on the constructive scattering or diffraction from different elements (such as metal corrugations or nanorings) of the nanostructures. However, subwavelength-size nanoholes, without nearby nanoscale features, results in an angularly dispersed emission from the distal surface.

Label-free surface-sensitive photonic microscopy with high spatial resolution using azimuthal rotation illumination.

Surface plasmon resonance microscopy (SPRM) with single-direction illumination is a powerful platform for biomedical imaging because of its wide-field, label-free, and high-surface-sensitivity imaging capabilities. However, two disadvantages prevent wider use of SPRM. The first is its poor spatial resolution that can be as large as several micrometers.

Fano resonance and polarization transformation induced by interpolarization coupling of Bloch surface waves.

In this work, the resonant coupling behaviors between the transverse-electric (TE) and transverse-magnetic (TM) Bloch surface waves (BSWs) on a dielectric multilayer have been theoretically studied. Due to the different penetration depths in the dielectric multilayer, the TM BSWs and TE BSWs can act as the radiative and dark electromagnetic modes, respectively. By using a rectangular grating on the dielectric multilayer, both Rabi splitting and Fano resonance phenomena based on the coupling of the two BSW modes were demonstrated, through tuning the period of the grating and the azimuthal angle of the incoming wave.

Two-Dimensional Photonic Devices based on Bloch Surface Waves with One-Dimensional Grooves.

Both experiments and simulations show that the polarization state and propagation path of the Bloch surface waves sustained on a dielectric multilayer, can be manipulated with the grooves inscribed on this multilayer. These grooves can be easily producible, accessible and controllable. Various nano-devices for the Bloch surface waves, such as the launcher, beam splitter, reflector, polarization rotator, and even the photonic single-pole double-throw switch, were all experimentally realized with the properly designed grooves, which are consistent with the numerical simulations.

A superior bright NIR luminescent nanoparticle preparation and indicating calcium signaling detection in cells and small animals.

Background: Near-field fluorescence (NFF) effects were employed to develop a novel near-infrared (NIR) luminescent nanoparticle (LNP) with superior brightness. The LNP is used as imaging contrast agent for cellular and small animal imaging and furthermore suggested to use for detecting voltage-sensitive calcium in living cells and animals with high sensitivity.

Results: NIR Indocyanine green (ICG) dye was conjugated with human serum albumin (HSA) followed by covalently binding to gold nanorod (AuNR).

Blocking TIR Domain Interactions in TLR9 Signaling.

Interaction of TLR9 with ligands activates NF-κB, leading to proinflammatory cytokine production. Excessive TLR activation is a pathogenic factor for inflammatory diseases. This study has examined cell-permeating decoy peptides (CPDPs) derived from the TLR9 Toll/IL-1R resistance (TIR) domain.

Glucose-sensitive silicone hydrogel contact lens toward tear glucose monitoring.

Accurate and reliable monitoring of blood glucose is needed for the treatment of diabetes, which has many challenges, including lack of patient compliance. Measuring tear glucose is an alternative to traditional finger-stick tests used to track blood sugar levels, but glucose sensing using tears has yet to be achieved. We report a methodology for possible tear glucose monitoring using glucose-sensitive silicone hydrogel (SiHG) contact lenses, the primary type of lenses available in today's market.