Lycopene Accumulation in Transgenic Mice Lacking One or Both Carotenoid Cleaving Enzymes.

Background: β-carotene oxygenase 1 (BCO1) and β-carotene oxygenase 2 (BCO2) are responsible for the cleavage of carotenoids in mammals.

Objective: The goals of this study were to (1) establish the relative contribution of each enzyme on lycopene accumulation in mice and (2) examine the role of lycopene on gene expression in the gut of wild type (WT) mice.

Methods: We utilized male and female WT, Bco1, Bco2, and Bco1Bco2 double knockout (DKO) mice.

Iron Deficiency and Overload Modulate the Inflammatory Responses and Metabolism of Alveolar Macrophages.

Alveolar macrophages (AM) are critical to defense against respiratory pathogens. This study evaluated cellular iron imbalance to immunometabolism in endotoxin-polarized porcine AMs (PAMs). PAMs collected from five 6-week-old pigs were treated with a basal media, iron chelator, or ferric ammonium citrate to maintain iron replete or induce iron deficiency or overload, respectively.