Prospective evaluation of accuracy of HIV viral load monitoring using the Aptima HIV Quant Dx assay with fingerstick and venous dried blood spots prepared under field conditions in Kenya.

Quantification of HIV-1 RNA is essential for clinical management of HIV patients. The limited throughput and significant hands-on time required by most HIV Viral load (VL) tests makes it challenging for laboratories with high test volume, to turn around patient results quickly. The Hologic Aptima HIV-1 Quant Dx Assay (Aptima), has the potential to alleviate this burden as it is high throughput and fully automated.

Performance evaluation of the Aptima HIV-1 Quant Dx assay for detection of HIV in infants in Kenya.

Background: Early infant diagnosis (EID) of HIV-1 exposed infants enables timely initiation of antiretroviral therapy (ART), thereby allowing early diagnosis and treatment to slow disease progression and reduce mortality. Turn-around time to results, partially caused by low to medium throughput technology, remains a hindrance to early treatment. A major solution to this challenge is to incorporate high throughput and accurate technologies in the testing process.

Performance and usability of mPIMA™ HIV 1/2 viral load test in point of care settings in Kenya.

Background: HIV viral load testing is the standard of care for monitoring antiretroviral therapy. In resource-limited settings such as Kenya, access to HIV viral load monitoring is suboptimal due to reliance on centralized laboratory based in vitro diagnostics. Point of care technologies have the potential to improve access and reduce test to result turnaround time.

On-demand controlled release of anti-inflammatory and analgesic drugs from conducting polymer films to aid in wound healing.

An electronically-controlled drug delivery system (eDDS) for the on-demand release of anti-inflammatory, anti-microbial and analgesic agents to aid in wound healing is currently under development. The loading of several drugs into conductive polymer films and their subsequent on-demand, controlled release upon application of an electrical potential to the polymer film has been demonstrated. The loading and release (active and passive) of Ibuprofen sodium salt - a negatively charged analgesic and anti-inflammatory agent - from polypyrrole films is described.