Publications by authors named "Joseph M C Lee"
Article Synopsis
- Transcription by RNA Polymerase II (Pol II) relies on the formation of a pre-initiation complex at the promoter, traditionally seen as dependent on the TATA-box binding protein (TBP).
- Acute depletion of TBP in mouse embryonic stem cells does not significantly impact ongoing Pol II transcription, indicating a TBP-independent mechanism for transcriptional initiation.
- While TBP affects RNA Polymerase III initiation, the TFIID complex remains functional with reduced factors, allowing Pol II transcription to continue efficiently without TBP.
Membrane-induced amphipathic helices (m-AH) can act as membrane curvature sensors by binding preferentially to hydrophobic lipid packing defects enriched in curved surfaces. Reliance on hydrophobicity and membrane curvature for binding is enhanced when electrostatic interactions are weak. We probed the role of modifying membrane and protein charge on the curvature sensing of two m-AH-containing proteins, CTP:phosphocholine cytidylyltransferase (CCT) and α-synuclein (α-syn).
View Article and Find Full Text PDFCTP:phosphocholine cytidylyltransferase (CCT) is an amphitropic protein regulating phosphatidylcholine synthesis. Lipid-induced folding of its amphipathic helical (AH) membrane-binding domain activates the enzyme. In this study we examined the membrane deforming property of CCT in vitro by monitoring conversion of vesicles to tubules, using transmission electron microscopy.
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