Superficially porous particles with 1000Å pores for large biomolecule high performance liquid chromatography and polymer size exclusion chromatography.

To facilitate mass transport and column efficiency, solutes must have free access to particle pores to facilitate interactions with the stationary phase. To ensure this feature, particles should be used for HPLC separations which have pores sufficiently large to accommodate the solute without restricted diffusion. This paper describes the design and properties of superficially porous (also called Fused-Core, core shell or porous shell) particles with very large (1000Å) pores specifically developed for separating very large biomolecules and polymers.

Are sub-2 μm particles best for separating small molecules? An alternative.

Superficially porous particles (SPP) in the 2.5-2.7 μm range provide almost the same efficiency and resolution of sub-2 μm totally porous particles (TPP), but at one-half to one-third of the operating pressure.

Optimized superficially porous particles for protein separations.

Continuing interest in larger therapeutic molecules by pharmaceutical and biotech companies provides the need for improved tools for examining these molecules both during the discovery phase and later during quality control. To meet this need, larger pore superficially porous particles with appropriate surface properties (Fused-Core(®) particles) have been developed with a pore size of 400 Å, allowing large molecules (<500 kDa) unrestricted access to the bonded phase. In addition, a particle size (3.

Fused-core particle technology in high-performance liquid chromatography: An overview.

The advent of superficially porous particles (SPPs) for packed HPLC columns has changed the way that many practitioners have approached the problem of developing needed separations. The very high efficiency of such columns, combined with convenient operating conditions, modest back pressures and the ability to use conventional HPLC instruments has resulted in intense basic studies of SPP technology, and widespread applications in many sciences. This report contains an overview of the SPP technology first developed in 2006 by Advanced Materials Technology, Inc.

Superficially porous silica particles with wide pores for biomacromolecular separations.

Since 2006, columns of superficially porous particles (SPPs), often called Fused-core(®), porous-shell or core-shell particles, have had serious impact on HPLC separations. These particles have pore diameters of about 100Å designed for separating small molecules. More recently, SPPs with 160-200Å pore diameter have been made available for separating peptides and small proteins.

Performance characteristics of new superficially porous particles.

Superficially porous particles (also called Fused-Core, core shell or porous shell particles) show distinct advantages over comparable totally porous particles for separating small molecules. Columns of Fused-Core particles exhibit very high efficiency because of superior eddy dispersion properties (smaller van Deemter A term). The efficiency for columns of 2.

Fast high performance liquid chromatography separations for proteomic applications using Fused-Core® silica particles.

The separation range of superficially porous particles (Fused-Core®) has been extended by design of particles with 160 Å pores. These particles show superior kinetics (lower resistance to mass transfer), allowing fast separations of peptides and small proteins (molecular weights of <15,000). The high efficiency and relatively low back pressure of these 2.