Publications by authors named "Joseph D Handen"
Deep UV Resonance Raman Spectroscopy for Characterizing Amyloid Aggregation.
Methods Mol Biol
July 2016
Article Synopsis
- Deep UV resonance Raman spectroscopy is an effective method for analyzing protein fibrils, overcoming challenges like low solubility and noncrystalline arrangements.
- This technique allows for selective enhancement of various chromophores in protein fibrils, providing insights into their structure and formation mechanisms.
- Advanced methods such as hydrogen-deuterium exchange and chemometrics can further detail the fibril core structure and protein interactions during fibril formation.
Article Synopsis
- The first measurement of vibrational circular dichroism (VCD) spectra for microcrystals of fibril-forming peptides, specifically human islet amyloid polypeptide (IAPP), was conducted.
- The study compared the VCD spectra of microcrystals and fibrils from the same peptide to gather insights into their structural characteristics and supramolecular chirality.
- It was found that enhanced VCD in these structures does not depend on the twisting of multiple filaments, which is a characteristic of fibrils but not microcrystals.