Mutation of a conserved glycine in the SH1-SH2 helix affects the load-dependent kinetics of myosin.

The ATP hydrolysis rate and shortening velocity of muscle are load-dependent. At the molecular level, myosin generates force and motion by coupling ATP hydrolysis to lever arm rotation. When a laser trap was used to apply load to single heads of expressed smooth muscle myosin (S1), the ADP release kinetics accelerated with an assistive load and slowed with a resistive load; however, ATP binding was mostly unaffected.

Slip sliding away: load-dependence of velocity generated by skeletal muscle myosin molecules in the laser trap.

Skeletal muscle's ability to shorten and lengthen against a load is a fundamental property, presumably reflecting the inherent load-dependence of the myosin molecular motor. Here we report the velocity of a single actin filament translocated by a mini-ensemble of skeletal myosin approximately 8 heads under constant loads up to 15 pN in a laser trap assay. Actin filament velocity decreased with increasing load hyberbolically, with unloaded velocity and stall force differing by a factor of 2 with [ATP] (30 vs.

A mutant heterodimeric myosin with one inactive head generates maximal displacement.

Each of the heads of the motor protein myosin II is capable of supporting motion. A previous report showed that double-headed myosin generates twice the displacement of single-headed myosin (Tyska, M.J.