Gas Tunnel Engineering of Prolyl Hydroxylase Reprograms Hypoxia Signaling in Cells.

Cells have evolved intricate mechanisms for recognizing and responding to changes in oxygen (O) concentrations. Here, we have reprogrammed cellular hypoxia (low O) signaling via gas tunnel engineering of prolyl hydroxylase 2 (PHD2), a non-heme iron dependent O sensor. Using computational modeling and protein engineering techniques, we identify a gas tunnel and critical residues therein that limit the flow of O to PHD2's catalytic core.

Gas tunnel engineering of prolyl hydroxylase reprograms hypoxia signaling in cells.

Cells have evolved intricate mechanisms for recognizing and responding to changes in oxygen (O) concentrations. Here, we have reprogrammed cellular hypoxia (low O) signaling via gas tunnel engineering of prolyl hydroxylase 2 (PHD2), a non-heme iron dependent O sensor. Using computational modeling and protein engineering techniques, we identify a gas tunnel and critical residues therein that limit the flow of O to PHD2's catalytic core.