Publications by authors named "Josef H Scheidt"
Optimization of Synthesis of the Amino Lipid ECO for Effective Delivery of Nucleic Acids.
Pharmaceuticals (Basel)
October 2021
Nucleic acids are promising for a variety of therapies, such as cancer therapy and the gene therapy of genetic disorders. The therapeutic efficacy of nucleic acids is reliant on the ability of their efficient delivery to the cytosol of the target cells. Amino lipids have been developed to aid in the cytosolic delivery of nucleic acids.
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- The study examines the use of targeted nanoparticles delivering ECO/miR-200c to alter the tumor microenvironment and treat triple-negative breast cancer (TNBC), utilizing non-invasive magnetic resonance molecular imaging (MRMI).
- Experiments involved transfecting TNBC cells with the nanoparticles and assessing their effect on tumor growth in mice models, showing significant suppression of tumor progression without harmful side effects.
- Results indicated that MRMI effectively tracked changes in tumor characteristics and highlighted the therapeutic impact of miR-200c by reducing EDB-FN expression, suggesting a promising approach for managing TNBC.
Nanoparticle based siRNA formulations often suffer from aggregation and loss of function during storage. We in this study report a frozen targeted RGD-polyethylene glycol (PEG)-ECO/siβ3 nanoparticle formulation with a prolonged shelf life and preserved nanoparticle functionality. The targeted RGD-PEG-ECO/siβ3 nanoparticles are formed by step-wised self-assembly of RGD-PEG-maleimide, ECO, and siRNA.
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