Evaluation of antimicrobial and antiproliferative activities of Actinobacteria isolated from the saline lagoons of northwestern Peru.

Extreme environments Morrope and Bayovar Salt lagoons, several ecosystems and microhabitats remain unexplored, and little is known about the diversity of Actinobacteria. We suggest that the endemic bacteria present in this extreme environment is a source of active molecules with anticancer, antimicrobial, and antiparasitic properties. Using phenotypic and genotypic characterization techniques, including 16S rRNA sequencing, we identified these bacteria as members of the genera Streptomyces, Pseudonocardia, Staphylococcus, Bacillus, and Pseudomonas.

The Lulun Project's social marketing strategy in a trial to introduce eggs during complementary feeding in Ecuador.

The Lulun Project incorporated a social marketing strategy that accompanied a randomized controlled trial (RCT) of a food-based intervention that introduced eggs into the complementary feeding diet of Ecuadorian infants. This strategy was designed to promote behaviour change, in this case, egg consumption, through voluntary prosocial behaviour, empowerment, and brand loyalty. A three-phase social marketing strategy (design, campaigns, and evaluation) contributed to our successful RTC by applying techniques drawn from marketing, publicity, design, and communications.

Psychometric properties of the Purpose-In-Life Test and age-related differences among women diagnosed with eating disorders.

The aim of this study was to analyze the psychometric properties of the Purpose-In-Life Test (PIL), as well as the age-related differences in meaning in life in women diagnosed with eating disorders. Participants were 250 Spanish women diagnosed with eating disorders who ranged from 12 to 60 years old. Confirmatory Factor Analysis, descriptive analyses, estimation of the internal consistency of the PIL, correlations between the PIL and the Beck Hopelessness Scale (BHS), Overweight Preoccupation Scale (OPS), and Body Investment Scale (BIS), and age differences were calculated.

Berberine as a photosensitizing agent for antitumoral photodynamic therapy: Insights into its association to low density lipoproteins.

Recent years have seen a growing interest in Berberine, a phytochemical with multispectrum therapeutic activities, as anti-tumoral agent for photodynamic therapy (PDT). In this context, low density lipoproteins (LDL) play a key role in the delivery of the photosensitizer in tumor cells. We correlate the physicochemical parameters of the berberine association to LDL with the influence of LDL-delivery on its accumulation in a glioma cell line and on its photo-induced activity in view of antitumor PDT.

Risk of recurrence of non-metastatic breast cancer in women under 40 years: a population-registry cancer study in a European country.

Unlabelled: Breast cancer in young patients is relatively uncommon. There is no consensus about the impact of young age on prognosis. The aim of this study was to analyze the effect of young age over the risk of recurrence of breast cancer using a population-registry cancer database in Spain.

Vascular anatomy of the dorsum of the foot.

Background: The authors' purpose was to study the anatomy of the dorsum of the foot and determine the distribution and caliber of all vascular branches that nourish the skin and the extensor hallucis longus and extensor digitorum longus tendons.

Methods: The authors performed 20 cadaver dissections. The skin paddle was designed within the dorsum of the foot.

Effective affinity distribution for the binding of metal ions to a generic fulvic acid in natural waters.

The effective distribution of affinities (Conditional Affinity Spectrum, CAS) seen by a metal ion binding to a humic substance under natural water conditions is derived and discussed within the NICA-Donnan model. Analytical expressions for the average affinity of these distributions in general multi-ion mixtures are reported here for the first time. These expressions enable a simple evaluation of the effect of all interfering cations on the affinity distribution of a given one.

Laparoscopic kidney transplantation.

We present the details of the first laparoscopic transplantation of a kidney from a living, related donor, performed April 16, 2009. Surgical and functional results were acceptable. Surgical time was 240 min (53 min for vascular suture), with blood loss of 300 cm(3) and a hospital stay of 14 d.

Competitive Cd2+/H+ complexation to polyacrylic acid described by the stepwise and intrinsic stability constants.

Stepwise constants can be used to describe competitive proton and metal binding to macromolecules with a large number of sites. With the aim of accessing information on the microscopic binding model, we report an expression that connects the stepwise constants to the site-specific metal constants. This expression holds for a very general complexation model including heterogeneity, interactions, and chelate complexation.

Competitive ion complexation to polyelectrolytes: determination of the stepwise stability constants. The Ca2+/H+/polyacrylate system.

This work presents a new methodology aimed at obtaining the stepwise stability constants corresponding to the binding of ions (or other small molecules) to macromolecular ligands having a large number of sites. For complexing agents with a large number of sites, very simple expressions for the stepwise stability constants arise. Such expressions are model-independent; that is, they allow the determination of the stepwise stability constants without making any previous assumption of the detailed complexation mechanism.

Ligand mixture effects in metal complex lability.

The degree of lability of a given metal complex species is modified in the presence of a mixture of ligands. This modification is a consequence of the coupling of the association and dissociation processes of all of the complexes according to the competitive complexation reaction scheme. We show that, because of the mixture effect, the lability of a given complex usually increases when another more labile complex is added into the system, while it decreases upon addition of a less labile one.

Lability of a mixture of metal complexes under steady-state planar diffusion in a finite domain.

The rigorous analytical solution for the fluxes from a mixture of 1:1 metal complexes toward an active surface under steady-state planar diffusion in a finite domain and excess ligand conditions allows for the computation of the global degree of lability of the system as well as particular degrees of lability of each complex in the mixture. This kind of system is found in a variety of fields ranging from electrochemical techniques (such as stripping chronopotentiometry at scanned deposition potential, SSCP) to analytical devices (such as diffusion gradients in thin-film gels, DGT). Among the specific effects arising from the presence of a mixture of ligands competing for the metal we highlight the following: (i) The degree of lability of a complex in the mixture differs from its degree of lability in an unmixed system with the same ligand concentration, and (ii) the degree of lability of one complex depends on (i.

Lability criteria for successive metal complexes in steady-state planar diffusion.

The lability of sequential metal complexes, ML, ML2, ML3, ...

[Intestinal hemangiopericytoma].

A case of intestinal hemangiopericytoma is described. This localization is extremely rare. Clinical presentation consisted of upper digestive hemorrhage and abdominal mass; 9 months after surgical removal, the patient presented a single liver metastasis located in the left lobe, which was resected.

[GESIDA/GESITRA-SEIMC, PNS and ONT consensus document on solid organ transplant (SOT) in HIV-infected patients in Spain (March, 2005)].

Solid organ transplant may be the only therapeutic alternative in some HIV-infected patients. Experience in North America and Europe during the last five years shows that survival at three years after an organ transplant is similar to that observed in HIV-negative patients. The criteria agreed upon to select HIV patients for transplant are: no opportunistic infections (except tuberculosis, oesophageal candidiasis or P.

The development of laparoscopic surgery in Spain.

Aim: To assess degree of development and level of acceptance of laparoscopic surgery in Spain.

Method: A questionnaire was sent to all members of the Spanish Association of Surgeons in April 2003. It included 32 questions, 9 of which were general, and 23 referred to specific clinical situations, techniques, and standard practice.

[Bilateral laparoscopic nephrectomy for renal cancer].

Objectives: To report the case of a patient with a bilateral synchronic renal tumor who underwent a laparoscopic surgical approach.

Methods: 57-year-old caucasian male with history of end stage renal disease and secondary hypertension, type II diabetes Mellitus, proteinuria of 2.83 g/24 hours, chronic smoker, and allergic to iodinated contrast who underwent right nephrectomy and simultaneous left laparoscopic transperitoneal nephroureterectomy for a bilateral renal tumor.

Phase I pharmacokinetic study of a single intravesical instillation of gemcitabine administered immediately after transurethral resection plus multiple random biopsies in patients with superficial bladder cancer.

Purpose: In this phase I study we determined the pharmacokinetic and toxicity profiles of a single intravesical instillation of gemcitabine administered immediately after complete transurethral resection (TUR) plus multiple random biopsies.

Materials And Methods: Ten patients with superficial bladder cancer clinically staged as Ta/T1 with no carcinoma in situ were included. A single dose of gemcitabine was administered intra-vesically immediately after TUR plus 6 random biopsies.

Percutaneous nephroscopic management of upper urinary tract transitional cell carcinoma: recurrence and long-term followup.

Purpose: We present long-term results of the percutaneous approach and resection of upper urinary tract transitional cell carcinoma, and we evaluate the prognostic factors related to recurrence.

Materials And Methods: A total of 34 patients underwent primary percutaneous resection of an upper urothelial tumor. We treated the patients with a superficial tumor that was completely resected macroscopically.

Mucosal fold as a mechanical cause of obstruction in orthotopic ileal neobladder.

Studer's ortothopic neobladder after radical cistectomy has become a good alternative and has improved the patients' quality of life. Voiding dysfunction after surgery is one of the possible late complications with incomplete emptying of the neobladder and chronical urinary retention. The mechanical obstruction may be due to recurrence of the tumor, anterior vaginal wall prolapse, urethrointestinal anastomosis stricture, mucose plug, stone and mucosal fold.

[Overview of the results of the treatment of transitional cell carcinoma by ureteroscopy].

Conservative treatment of the transitional cell carcinoma of the upper urinary tract by ureteroscopy is currently a therapeutic approach which is accepted by urologists. Recurrences are frequent (0-65%), as well as in bladder tumors; the longer the follow-up of the series the higher the recurrence. Multifocality, history of bladder tumor or renal pelvis localization are prognostic factors for recurrence.

[Protocol for ureteroscopy in the diagnosis and treatment of the upper urinary tract transitional cell carcinoma].

Since Pérez-Castro and Martínez-Piñeiro initiated diagnostic and therapeutic ureteroscopy this technique has gained a place in the management of upper urinary tract tumors. Improvement of the equipment (rigid and flexible), better diagnosis and knowledge of outcomes and allows to treat a group of patients with transitional cell carcinoma of the ureter and pelvis by the conservative retrograde technique. In this article, we present an overview of indications and management of the upper urinary tract tumor by ureteroscopy.