Lyophilized Autologous Serum Eyedrops: Experimental and Comparative Study.

Purpose: To analyze the biological stability of autologous serum eyedrops after lyophilization.

Design: Prospective, comparative experimental study.

Methods: This was a comparative study with serum obtained from 12 healthy volunteers.

Changes in Corneal Expression of after Autologous Serum Eyedrop Treatment in Patients with Limbal Stem Cell Deficiency.

: To evaluate the effect of autologous serum eyedrops treatment on corneal expression of the in patients with limbal deficiency. : A prospective and comparative interventional case series study of 42 eyes of 21 patients was performed before and 8 weeks after treatment with autologous serum. All patients underwent a complete ophthalmic assessment of the tear film and ocular surface, corneal impression cytology (IC) and detection by Reverse Transcription-Polymerase Chain Reaction (RT-PCR).

Modified lateral tarsal strip for involutional entropion and ectropion surgery.

Purpose: Our purpose was to assess our outcomes in involutional lower eyelid ectropion and entropion surgery after horizontal eyelid tightening by a modified tarsal strip technique.

Methods: A prospective study of 88 eyelids with ectropion and 96 with entropion was performed. Patients were randomly distributed into two groups: the control group included 90 eyelids (46 entropion and 44 ectropion) and the other group of 94 eyelids (50 entropion and 44 ectropion).

Effects of Autologous Serum Eye Drops on Conjunctival Expression of MUC5AC in Patients With Ocular Surface Disorders.

Purpose: To assess the effects of autologous serum eye drops on conjunctival expression of the mucin gene MUC5AC by means of quantitative reverse transcription polymerase chain reaction (RT-qPCR).

Methods: A prospective and comparative interventional case series study of 38 eyes of 19 patients with different ocular surface disorders was performed before and 6 weeks after the treatment with autologous serum eye drops. All patients underwent a complete ophthalmic assessment, including evaluation of the tear film, ocular surface exploration, conjunctival impression cytology (IC), and MUC5AC detection by reverse transcription polymerase chain reaction.

Stability of Growth Factors in Autologous Serum Eyedrops After Long-Term Storage.

Purpose: The purpose of this study is to assess the stability of the growth factors (GF) in autologous serum eyedrops under different storage conditions.

Methods: The concentration of epidermal growth factor (EGF), transforming growth factor-β (TGF-β1), platelet-derived growth factor AB (PDGF-AB), and albumin was measured in fresh and defrosted samples of autologous serum under different storage conditions. The fresh and defrosted samples were cooled at 4 °C, and they were studied immediately after preparation, or after defrosting, and after 1, 2, 3, and 4 weeks.

Amniotic membrane transplantation in acute toxic epidermal necrolysis: histopathologic changes and ocular surface features after 1-year follow-up.

Purpose: To evaluate ocular features and histopathologic changes in patients with toxic epidermal necrolysis (TEN) treated with amniotic membrane transplantation (AMT) in the acute phase.

Methods: A prospective study of 5 eyes (3 patients) with severe ocular involvement and 4 eyes (2 patients) with moderate involvement treated with AMT in the acute phase of TEN was performed. Patients underwent a complete ophthalmic assessment before the intervention and 3, 6, 9, and 12 months postoperatively.

Autologous serum eye drops diluted with sodium hyaluronate: clinical and experimental comparative study.

Purpose: To assess the efficacy of sodium hyaluronate as vehicle for diluting autologous serum.

Methods: The concentration and temporal stability of EGF, TGF-β, PDGF-AB and albumin in fresh and frozen samples of autologous serum diluted with sodium hyaluronate and saline solution, as well as the pH, osmolarity and density was studied. In parallel, the clinic effects of autologous serum diluted to 20% with sodium hyaluronate were compared with another solution of autologous serum diluted to 20% with saline in a prospective, comparative, randomized and double-blind study in 26 patients (52 eyes) with Sjögren syndrome.

Use of containers with sterilizing filter in autologous serum eyedrops.

Objective: To assess the effect of the use of containers with an adapted sterilizing filter on the contamination of autologous serum eyedrops.

Design: Prospective, consecutive, comparative, and randomized study.

Participants: Thirty patients with Sjögren syndrome.

Ocular features and histopathologic changes during follow-up of toxic epidermal necrolysis.

Objective: To evaluate ocular surface histopathologic changes and ocular sequelae in the follow-up of patients with toxic epidermal necrolysis (TEN).

Design: Prospective, consecutive, comparative, interventional case series.

Participants: Eleven patients (22 eyes) with TEN that developed after drug treatment and 33 normal subjects as the control group.

Histopathologic limbus evolution after alkaline burns.

Purpose: To study the histopathologic evolution of the corneal limbus after alkaline burns according to the clinical severity and therapy used.

Methods: A prospective study of 15 eyes from 12 patients (9 men and 3 women) with moderate and severe alkaline burns was performed. All patients were divided into 2 groups in accordance with the clinical ocular severity and the therapy that was used: medical therapy, amniotic membrane transplantation (AMT), autologous limbal transplantation (ALT), and ALT combined with AMT (ALT + AMT).