Advances in chemical and biological characterization of triterpenoid saponins from L. using UHPLC-MS/MS and cell-based assays.

A fraction enriched in triterpenoid saponins (F4) from L. was chemically characterized by UHPLC-ESI-QTOF-MS/MS and NMR analyses. The results proposed the presence of nine monodesmosidic saponins derived from oleanolic acid, including two reported for the first time for this species, : 3-O-{β-D-glucopyranosyl (1→4)-[β-D-xylopyranosyl (1→2)]-β-D-glucopyranosyl (1→4)-[β-D-glucopyranosyl (1→2)]-α-L-arabinopyranosyl}-13β, 28-epoxy- 22 acetyl- 28 methoxy-16α, 24-oleananediol and : 3-O-{β-D-glucopyranosyl (1→4)-β-D-glucopyranosyl (1→4)-[β-D-xylopyranosyl (1→2)]-β-D-glucopyranosyl (1→4)-[β-D-glucopyranosyl (1→2)]-α-L-arabinopyranosyl}-13β, 28-epoxy- 16α, 30-oleananediol.