The long non-coding RNA in the regulation of alternative protein-coding transcripts and in human breast cancer cells: implications to epigenetic therapy.

Alternative protein-coding transcripts of the gene have been associated with dual functions in human cancer: while isoform has oncogenic properties, is a tumour suppressor frequently silenced by hypermethylation. Recently, the antisense long non-coding RNA RASSF1 ( was implicated in a -specific mechanism for the epigenetic repression mediated by PRC2 (Polycomb Repressive Complex 2). Here, we evaluated the methylation patterns of the promoter regions of and and the expression levels of these transcripts in breast cancer and breast cancer cell lines.

Shorter CAG repeat in the AR gene is associated with atypical hyperplasia and breast carcinoma.

Background: Previous reports into the role of [CAG]n repeat lengths in the androgen receptor (AR) gene indicate that these may play an important part in the development and progression of breast cancer, however, knowledge regarding benign breast lesions is limited.

Patients And Methods: PCR-based GeneScan analysis was used to investigate the [CAG]n repeat length at exon 1 of the AR gene in 59 benign breast lesions (27 fibroadenomas, 18 atypical hyperplasias, and 14 hyperplasias without atypia) and 54 ductal breast carcinomas. Seventy-two cancer-free women were used as a control group.

Gene expression profile associated with response to doxorubicin-based therapy in breast cancer.

Purpose: This study was designed to identify genes that could predict response to doxorubicin-based primary chemotherapy in breast cancer patients.

Experimental Design: Biopsy samples were obtained before primary treatment with doxorubicin and cyclophosphamide. RNA was extracted and amplified and gene expression was analyzed using cDNA microarrays.