Publications by authors named "Jose R F Alves-Junior"

Background: The hematology of turtles of the Amazon, such as the Arrau turtle (Podocnemis expansa), has not been well described in the literature. Referencing how to collect and analyze blood samples is fundamental for the medical practice of these exotic animals.

Objectives: The aim of this study was to evaluate the hematologic parameters of Arrau turtles of the Amazon to determine the best anticoagulant (EDTA, citrate, or sodium heparin) and cytologic stain (Giemsa, Quick Panoptic, or Rosenfeld) for use with this species.

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Morphological and molecular techniques were used to investigate the presence of hemogregarines and haemosporidians in biological samples of free-living Geoffroy's side-necked turtles () and Giant Amazon turtles () from Brazil. No evolutionary form of haemosporidians or hemogregarines were observed in the blood smears of 83 samples, and there were no meronts in the histological sections of 31 necropsied samples. All DNA samples extracted from tissues and blood aliquots were negative in haemosporidian PCR assays (based on the mitochondrial cytochrome gene) and hemogregarine PCR assays (based on the 18S rRNA gene).

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Although Orinoco goose (Neochen jubata) is an anatid species widely distributed in South America, scarce are the reports on the occurrence of arthropod-borne pathogens in this avian species. The present work aimed to verify, by serological and molecular methods, the occurrence of haemosporida piroplasmids and Anaplasmataceae agents in wild Orinoco geese captured in Brazil. Between 2010 and 2014, 62 blood samples were collected from free-living geese captured in the Araguaia River, Goiás State, Brazil.

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Purpose: To evaluate the effects of different concentrations of an anesthetic association in giant amazon turtles (Podocnemis expansa).

Methods: Twenty healthy P. expansa of both sexes weighing between 1.

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In order to investigate new hosts for Anaplasmataceae agents in Brazil, we collected blood samples from 21 wild birds. Using molecular techniques, we detected the presence of Anaplasma phagocytophilum, Ehrlichia chaffeensis, and an Ehrlichia species closely related to Ehrlichia canis in carnivorous avian blood samples. In addition, an Ehrlichia species closely related to an Ehrlichia species found in wild felines in Brazil was also detected in a goose blood sample.

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