Differential gene expression between and maturation: a comparative study with bovine oocytes derived from the same donor pool.

Objective: maturation has been shown to influence gene expression in oocytes, but a common shortcoming in reports on the matter has been the use of different donors in each experimental group thus disregarding donor effects. This study aimed to investigate the abundance of mRNA in oocytes matured and obtained from the same group of donors.

Methods: A bovine model was used to assess the relative abundance of specific transcripts in in vitro-matured (IN VITRO-OPU) and -matured (IN VIVO-OPU) oocytes collected from the same donors by transvaginal ovum pick-up (OPU).