Undisturbed culture: a clinical examination of this culture strategy on embryo in vitro development and clinical outcomes.

Objective: To compare the effect of a fully undisturbed culture strategy over a sequential one on embryo in vitro development and clinical outcomes in intracytoplasmic sperm injection (ICSI) cycles.

Design: Retrospective cohort study.

Setting: University-affiliated private IVF center.

Elevated serum progesterone does not impact euploidy rates in PGT-A patients.

Purpose: Some women undergoing stimulated cycles have elevated serum progesterone (P) on the day of ovulation trigger, but its effect on embryo quality is unclear. We analyze embryo quality among patients with high and low serum P undergoing preimplantation genetic testing for aneuploidy (PGT-A).

Methods: This retrospective study included 1597 patients divided into two groups by serum P values: < 1.

The influence of oxygen concentration during embryo culture on obstetric and neonatal outcomes: a secondary analysis of a randomized controlled trial.

Study Question: Does oxygen concentration during 3-day embryo culture affect obstetric and neonatal outcomes?

Summary Answer: Oxygen concentration during 3-day embryo culture does not seem to affect the obstetric and neonatal outcomes measured.

What Is Known Already: Atmospheric oxygen appears to be harmful during extended embryo culture. Embryo culture conditions might therefore be a potential risk factor for subsequent fetal development and the health of future children.

Effect of oocyte vitrification on embryo quality: time-lapse analysis and morphokinetic evaluation.

Objective: To analyze whether oocyte vitrification may affect subsequent embryo development from a morphokinetic standpoint by means of time-lapse imaging.

Design: Observational cohort study.

Setting: University-affiliated private IVF center.

Combination of metabolism measurement and a time-lapse system provides an embryo selection method based on oxygen uptake and chronology of cytokinesis timing.

Objective: To evaluate correlations between oxygen consumption (OC) measurements before and after embryo cytokinesis, observing OC during embryo cleavages and combining that information with morphokinetics to relate to implantation potential.

Design: Prospective cohort study.

Setting: University-affiliated private IVF unit.

The Metabolomic Profile of Spent Culture Media from Day-3 Human Embryos Cultured under Low Oxygen Tension.

Despite efforts made to improve the in vitro embryo culture conditions used during assisted reproduction procedures, human embryos must adapt to different in vitro oxygen concentrations and the new metabolic milieu provided by the diverse culture media used for such protocols. It has been shown that the embryo culture environment can affect not only cellular metabolism, but also gene expression in different species of mammalian embryos. Therefore we wanted to compare the metabolic footprint left by human cleavage-stage embryos under two types of oxygen atmospheric culture conditions (6% and 20% O2).

Outcome of cryotransfer of embryos developed from vitrified oocytes: double vitrification has no impact on delivery rates.

Objective: Evaluate the outcome of cryotransfer of embryos developed from vitrified oocytes.

Design: Retrospective cohort study.

Setting: Private university-affiliated IVF center.