Publications by authors named "Jose J Lira-Amaya"
Canine monocytic ehrlichiosis (CME) is the most common tick-borne disease affecting domestic dogs and other wild canids. It has a worldwide distribution and is associated with the presence of the brown dog tick. Few studies have been conducted in Mexico to identify and characterize genetic variability.
View Article and Find Full Text PDFTwo hundred and thirty-three blood samples of water buffalo were collected on four farms in Veracruz state and Tabasco state, Mexico, to detect and confirm the identities of and spp. sequences. Nested PCR assays were used for the amplification of specific genes encoding rhoptry-associated protein (RAP-1), I-I restriction fragment, and major surface protein 5 (MSP5).
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- Tick-borne bacterial pathogens (TBBPs) pose significant public health risks worldwide, particularly affecting dogs and occasionally humans.
- The study identified the brown dog tick as the primary species affecting dogs in Juárez City, Mexico, accounting for 99.8% of cases and investigated TBBPs through DNA analysis from ticks and dog blood samples.
- This research provided the first molecular detection of specific pathogens in domestic dogs in Mexico, revealing co-detection of multiple pathogens in both ticks and dog samples, and highlighted the prevalence of adult female ticks, which may increase transmission risks.
Comparative Study of Indirect Fluorescent Antibody, ELISA, and Immunochromatography Tests for Serological Diagnosis of Bovine Babesiosis Caused by .
Animals (Basel)
November 2021
Article Synopsis
- The indirect fluorescent antibody test (IFAT) is the standard method for diagnosing bovine babesiosis, while this study aims to compare ELISA and ICT for serological diagnosis in cattle.
- The performance evaluation involved 30 positive and 30 negative sera, showing ELISA sensitivity at 87% and specificity at 80%, while ICT displayed slightly better sensitivity at 90% and specificity at 83.3%.
- Overall concordance with IFAT was high for both tests, but ICT demonstrated greater effectiveness, suggesting it could be a practical, field-friendly option for antibody detection without needing specialized lab equipment.
American bison () is listed as near-threatened and in danger of extinction in Mexico. Recent studies have demonstrated the presence of several emerging pathogens at the Janos Biosphere Reserve (JBR), inhabited by one wild herd of American bison. Blood samples were collected from 26 American bison in the JBR.
View Article and Find Full Text PDFThe causative agents of Babesiosis are intraerythrocytic protozoa of the genus . parasites are present around the world, affecting several mammals including humans, pets and livestock, hence its medical and veterinary relevance. spp.
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- Ehrlichia canis is a significant health concern in dogs, often causing high morbidity and mortality, and can be difficult to diagnose due to asymptomatic cases and cross-reactivity in serological tests.
- A study examined 59 euthanized dogs with ticks but no clinical symptoms, finding that while 52.55% tested negative for E. canis in blood samples, 61.30% were positive in tissue biopsies.
- Results indicated that E. canis DNA was present in various organs despite blood tests being negative, highlighting the limitations of blood PCR and the need for more comprehensive testing in tissues.
Babesia bigemina: Advances in continuous in vitro culture using serum-free medium supplemented with insulin, transferrin, selenite, and putrescine.
Parasitol Int
June 2018
Bovine babesiosis: Cattle protected in the field with a frozen vaccine containing Babesia bovis and Babesia bigemina cultured in vitro with a serum-free medium.
Parasitol Int
April 2018
An attenuated live vaccine containing Babesia bovis and B. bigemina cultured in vitro with a serum-free medium was assessed for its clinical protection conferred of naïve cattle, under natural tick-challenge in a high endemicity zone to Babesia spp. Three groups of six animals were treated as follows: group I (GI) received a vaccine derived from parasites cultured with a free-serum medium; group II (GII) were immunized with the standard vaccine, with parasites cultured in a medium supplemented with 40% (v/v) bovine serum; and a control group (GIII) inoculated with non-infected bovine erythrocytes.
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