Publications by authors named "Jose Damasceno"

Article Synopsis
  • - The study aimed to investigate the prevalence of piroplasmids in dogs and ticks in Teresópolis, Brazil, assessing their clinical and blood profiles through a combination of blood tests and PCR methods.
  • - Out of 400 dogs examined between 2020 and 2021, 2.3% tested positive for piroplasmids, with notable associated health issues including bleeding, jaundice, and specific blood cell irregularities; Rangelia vitalii was the primary infection found.
  • - The findings highlight that while two species of piroplasmids can infect dogs in the region, Rangelia vitalii is the most common in the studied population, indicating the need for awareness and potential treatment options
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Piroplasm infections in domestic cats have been reported with increasing frequency in numerous countries. However, in some states of Brazil, little information is available about the occurrence of these parasites. Blood samples were collected from 250 cats treated at a private clinic in the mountainous region of Rio de Janeiro.

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Purpose: This study aimed to analyze the frequency of piroplasmids in the blood of dogs in Rio de Janeiro, compare the performance of microscopic techniques, assess the risk factors associated with infections and also molecularly and morphologically characterize the piroplasmids identified.

Methods: In all, 407 blood samples were collected from dogs between 2018 and 2019. These were subjected to microscopic parasitological techniques for thin and thick smears, stained with Giemsa and using a rapid staining kit.

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Piroplasm species were analyzed by molecular tools in total 31 blood samples from positive dogs, previously checked by stained slides, stored until DNA extraction between 2016 to 2018 in the laboratory Clinical Analyzes in Niterói, Rio de Janeiro. The piroplasms were identified by PCR, targeting the 18S rRNA gene and sequencing. From the total number of samples only 24 (77.

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Corynebacterium ulcerans was isolated from nares of one asymptomatic dog kept in an animal shelter in the metropolitan area of Rio de Janeiro, Brazil. The RNA polymerase beta subunit-encoding gene was sequenced to confirm the species identity. C.

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