Association of oxygenation levels after successful mechanical thrombectomy under general anesthesia and 3-month functional outcome in patients with acute ischemic stroke: A prospective observational study.

Background: This study aimed to evaluate whether oxygenation levels (PaO <150 mmHg vs. PaO ≥150 mmHg) in mechanically ventilated patients with acute ischemic stroke (AIS) after successful endovascular treatment (EVT) under general anesthesia are associated with functional outcomes at three months.

Methods: We prospectively collected data over 30 months from patients with proximal occlusion of the anterior circulation treated with successful EVT under general anesthesia.

Molecular and biochemical analyses of germination of cowpea (Vigna unguiculata L.) seeds inhibited by n-propyl gallate reveal a key role of alternative oxidase in germination Re-establishment.

n-Propyl gallate (PG) is a phenolic compound that influences enzymatic processes, mostly involving AOX, PTOX, LOX, POD, and PPO. Here, analyses of different PG concentrations (1, 2.5, and 5 mM) during cowpea seed germination at 16, 32, and 48h showed that 2.

Clinical and genetic landscape of IRD in Portugal: pooled data from the nationwide IRD-PT registry.

This study aims to characterize the clinical spectrum and genetic landscape of IRDs in Portugal. Multicentre, cross-sectional, cohort study comprising consecutive patients with a clinical diagnosis of IRD and available genetic results, enroled in the IRD-PT registry (retina.com.

HiDaFernPT: Historical data of spore availability for 121 fern and lycopod taxa in Portugal (1926-2013).

Species phenology is being altered by ongoing climate changes with yet underappreciated consequences for ecological processes and ecosystem stability. Contrary to what happens with some key life events of flowering plants, comparatively little information exists about fern and lycophyte phenology and how it is affected by the current climatic changes. In part, this stems from the lack of long-term datasets.

Decoding Framework Dynamics in a Spin Crossover Flexible Metal-Organic Framework.

Functional spin crossover (SCO) metal-organic frameworks (MOFs) hold promise for miniaturized spin-based devices due to their tuneable molecule-based properties near room temperature. SCO describes the phenomenon where transition metal ions switch between high spin (HS) and low spin (LS) states upon external stimuli. However, even simple guest molecules like water can significantly alter the properties of these materials.

Comparison of fixed dose versus weight-adjusted heparin on the prevention of radial artery occlusion after diagnostic transradial catheterization.

Objectives: Transradial access (TRA) catheterization has demonstrated significant reductions in procedural complications compared with other access routes. However, radial artery occlusion (RAO) remains a concern, and the optimal dose of heparin to mitigate RAO has yet to be determined. This study aims to address this gap by investigating the impact of weight-adjusted heparin doses on the incidence of RAO in patients undergoing diagnostic transradial catheterization.

Sclerotherapy with low-dose 5% ethanolamine oleate in oral vascular anomalies: a retrospective study.

Objective: To describe the characteristics and outcomes of a cohort of patients with oral vascular anomalies (OVA) treated with sclerotherapy using 5% ethanolamine oleate at a dosage of 0.1 mL per 10 mm of lesion size.

Study Design: Sixty-seven patients were selected from a review of 300 medical records of individuals who had been diagnosed with OVA.

Population size, income and poor sanitation interact to explain widespread streamwater contamination by antidepressants in the Metropolitan Region of São Paulo.

The expansion of urban settlements over native environments may expose biodiversity to a host of emerging contaminants, with unintended ecological effects. This study evaluated patterns of contamination of streamwater by antidepressants in the Upper Tietê River Basin, a watershed of high social, economic and environmental relevance for comprising both the largest urban settlement in South America (the Metropolitan Region of São Paulo) and remnants of a globally important biodiversity hotspot (the Atlantic Rainforest). We sampled 53 third-order streams draining catchments regularly distributed across a gradient in urban cover.

Multicenter In-House Evaluation of an Amplicon-Based Next-Generation Sequencing Panel for Comprehensive Molecular Profiling.

Background: Predicting response to targeted cancer therapies increasingly relies on both simple and complex genetic biomarkers. Comprehensive genomic profiling using high-throughput assays must be evaluated for reproducibility and accuracy compared with existing methods.

Methods: This study is a multicenter evaluation of the Oncomine™ Comprehensive Assay Plus (OCA Plus) Pan-Cancer Research Panel for comprehensive genomic profiling of solid tumors.

Kidney Disease and Hematopoietic Stem Cell Transplantation.

Hematopoietic stem cell transplantation (HSCT) is a potentially curative therapy for patients with hematologic malignancies and certain solid tumors and nonmalignant hematologic conditions. Both AKI and CKD occur commonly after HSCT and are associated with significant morbidity and mortality. AKI and CKD in this setting may result from direct effects of the transplant or be caused by pretransplant bone marrow conditioning regimens and/or nephrotoxic agents administered in the post-transplant period.

Antioxidant treatment attenuates age-related placenta GLUT-1 and PLIN-2 downregulation.

Background And Aim: Pregnancy after the age of 35 is correlated with an increased risk of impaired placentation and the development of pregnancy-associated complications. Changes in uterine redox balance seem to play a role in these settings. In this work, we hypothesized that local redox dysregulation impacts the placenta metabolic profile.

Characterization and analysis of the bioactivity of the Piper rivinoides Kunth essential oil and its components myristicin and elemicin, against opportunistic fungal pathogens.

Candidiasis is an infectious disease caused by some fungi of the genus Candida. In Brazil, the incidence rate is higher than in European countries and the United States, and health problems occur mainly due to the virulence factors of the fungi, which have made treatment with commercial drugs difficult. Considering the context, plants rich in phenolic compounds, such as those of the genus Piper, have been studied due to their antimicrobial properties.

Safety and Efficacy of BioMime Sirolimus-Eluting Stent System in All-Comers Real-World Population With Coronary Artery Stenosis: MILES Global Registry.

Background: This study evaluated the safety and efficacy of BioMime sirolimus-eluting stent (SES) system, with an ultra-low strut thickness (65 µm), in real-world all-comers population with coronary artery stenosis (CAD).

Methods: This was a post-marketing, multicenter, single-arm, observational clinical registry among patients undergoing intervention for CAD. Patients were clinically followed up at 1, 9, 12, and 24 months after the index percutaneous coronary intervention.

Effect of the consumption of cashew nut (Anacardium occidentale l.) flour-based biscuits in overweight children: a pilot randomized clinical trial.

Background: cashew nut is an almond known for its cardiovascular benefits in adults and weight gain effects in malnourished children, as supported by research. However, its impact on overweight children remains unexplored.

Objective: to analyze the effect of consuming biscuits made with cashew nut flour on the blood glucose and serum triglyceride levels of children with overweight/obesity over four weeks.

Laticifers as in vivo plant cell factories for therapeutic proteins.

The demand for therapeutic proteins is growing annually. Novel approaches for the production of these molecules on a large scale are necessary, especially in plants. Plant laticifers could help provide an in vivo cell system for protein production expression that can reduce costs of production and downstream processing.

Electrochemical methods for the determination of acetaminophen in biological matrices: A critical review in the clinical field.

Background: Paracetamol or acetaminophen (APAP), or acetaminophen, is a widely used medication for pain relief and fever reduction due to its analgesic and antipyretic properties. However, excessive APAP consumption can lead to severe hepatotoxicity and nephrotoxicity, posing overdose risks. Consequently, the development of analytical methods for an accurate and rapid detection of APAP in biological matrices is of great interest in the health-related fields.

Polycystic liver - A cyst-ridden abdomen.

A 44-year-old female with an established diagnosis of autosomal dominant polycystic kidney disease (based on imaging findings and family history) was being evaluated for weight loss, early satiety and progressive abdominal distension. Kidney function had been relatively stable for years. Because of a history of multiple liver cysts on ultrasonography, the patient was referred for Gastroenterology for evaluation.

When disaster strikes: Conversion of unicompartmental knee arthroplasty to total knee arthroplasty.

Objectives: To investigate the causes of failure in unicompartmental knee arthroplasty (UKA), types of implants used in the revision, evaluate the need to use tibial stems, metal block augmentations, and bone grafts during conversion to total knee arthroplasty (TKA).

Methods: In a 10-year retrospective analysis, focussing on cases of UKA failure, our study aimed to categorise early and late failures, determine the primary failure modes and assess the utilisation of bone augmentations and grafts during conversion to TKA. We evaluated patient data, diagnoses, procedure intervals, and follow-up periods to provide a comprehensive understanding of the conversion process.

Phytochemical characterization, toxicity and pharmacological profile of the central effects of the fixed fruit pulp oil of Mauritia flexuosa L.F. (buriti).

Anxiety and depression are mental disorders that have been exponentially increasing over the last decades. Psychopharmacology emerged to try to alleviate the symptoms of these disorders; however, the side effects and the time it takes to achieve the desired effect are factors that decrease the search for and adherence to treatment. To remedy this situation, new compounds capable of improving the performance of these medications and reducing their adverse effects have been sought.

Anticonvulsant, Anticholinesterase and Cytoprotective Effects of the Aqueous Extract of Cham.

(1) Background: Cham is a Brazilian aromatic plant rich in phenolic compounds. In traditional medicine, its leaves are used to treat diseases of the Central Nervous System such as stress and anxiety. This study evaluates the capacity of the aqueous extract of as an anticonvulsant, anticholinesterase and antihemolytic agent.

Sustainable Synthesis of New Antioxidants from Hydroxytyrosol by Direct Biocatalytic Esterification in Ionic Liquids.

Hydroxytyrosol (HT) is a nutraceutical compound, mainly found in the fruit, leaves and waste from the olive oil industry, known for exhibiting one of the highest antioxidant activities among molecules of natural origin. To harness this bioactivity in cosmetics, pharmaceuticals and the food industry, it is essential to modify the hydrophilicity of HT to enhance its compatibility with lipid-based mixtures. This chemical modification must be carried out with high selectivity to avoid compromising its radical scavenging activity.

Metabolomics and proteomics in occupational medicine: a comprehensive systematic review.

Background: Occupational biomonitoring is essential for assessing health risks linked to workplace exposures. The use of 'omics' technologies, such as metabolomics and proteomics, has become crucial in detecting subtle biological alterations induced by occupational hazards, thereby opening novel avenues for biomarker discovery.

Aims: This systematic review aims to evaluate the application of metabolomics and proteomics in occupational health.

Environmentally friendly screen-printed electrodes for the selective detection of 4-bromo-2,5-dimethoxyphenethylamine (2C-B) in forensic analysis.

In response to the growing need for sustainable analytical methods, this study explores the repurposing of screen-printed electrodes (SPEs) that would otherwise be discarded. This involves recoating the working electrode surface with a graphite (Gr) and chitosan (CTS) dispersion, creating a reusable SPE (SPE-Gr/CTS). Demonstrating its utility, SPE-Gr/CTS was employed for the detection of 4-bromo-2,5-dimethoxyphenethylamine (2C-B), a phenylethylamine commonly used for recreational proposes.

Determination of drugs of abuse and metabolites in plasma microsamples by LC-MS/MS after microQuEChERS extraction.

Identifying drugs of abuse and their metabolites in plasma is vital in both forensic and clinical toxicology. While the QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) method offers an efficient approach to sample preparation, its application is complex due to the wide-ranging properties of target analytes and the challenges posed by biological matrix interferences. This study aims to develop a microQuEChERS approach for the quantification of 14 drugs of abuse and metabolites utilizing minimal sample and solvent volumes.