Publications by authors named "Jose Carlos"
Article Synopsis
- - The study investigates the genetic origins of eight hound-like dog breeds from the Mediterranean, exploring whether they share ancient common ancestors despite ongoing debates about dog domestication.
- - Microsatellite analysis shows low inbreeding values across all dog populations, while mitochondrial DNA results indicate prevalent haplogroups A and B, relationship links, and some traits resembling ancient Egyptian dogs.
- - Although there's no definitive evidence that these breeds are direct descendants of ancient Egyptian dogs, they exhibit strong morphological similarities and some genetic connections, highlighting the need for further research on their complex ancestry.
This work was performed to study Castilla Rose () as a potential source of polyphenols obtained by solid-state fermentation (SSF)-assisted extraction using the microorganism GH1 and to evaluate the antioxidant activity of the extracted compounds. First, water absorption capacity (WAC) of the plant material, radial growth of the microorganism, determination of best fermentation conditions, and maximum accumulation time of polyphenols were tested. Then, a larger-scale fermentation, polyphenols isolation by column liquid chromatography (Amberlite XAD-16) and recovered compounds identification by HPLC-MS were made.
View Article and Find Full Text PDFLipoxins are an important class of lipid mediators that induce the resolution of inflammation and arise from transcellular exchange of arachidonic acid (AA)-derived lipoxygenase products. Human epithelial 15-lipoxygenase-2 (h15-LOX-2), the major lipoxygenase in macrophages, has exhibited strict regiospecificity, catalyzing only the hydroperoxidation of carbon 15 of AA. To determine the catalytic potential of h15-LOX-2 in transcellular synthesis events, we reacted it with the three lipoxygenase-derived monohydroperoxy-eicosatetraenoic acids (HPETE) in humans: 5-HPETE, 12-HPETE, and 15-HPETE.
View Article and Find Full Text PDFSeveral strains of Thermus thermophilus were tested in order to detect purine nucleoside synthase activity using pyrimidine nucleosides as the sugar-donor and adenine or hypoxanthine as bases. High productivity values (t =1 hr) were obtained while completely avoiding adenosine-deaminase degradation of the products. N-2-deoxy-ribosyltransferase activity is described for the first time in hyperthermophilic bacteria.
View Article and Find Full Text PDFMost nemaline myopathy patients have mutations in the nebulin (NEB) or skeletal muscle alpha-actin (ACTA1) genes. Here we report for the first time three patients with severe nemaline myopathy and mutations of the ACTA1 stop codon: TAG>TAT (tyrosine), TAG>CAG (glutamine) and TAG>TGG (tryptophan). All three mutations will cause inclusion of an additional 47 amino acids, translated from the 3' UTR of the gene, into the mature actin protein.
View Article and Find Full Text PDFBackground: A retrospective study was carried out with all the cases of traumatic dislocation of the knee joint treated in our institution between 1988 and 1998.
Methods: In most cases (81%), the dislocation was reduced under general anesthesia and early surgical repair of damaged structures was performed as each case required. In five cases, the treatment was conservative because of associated skeletal and visceral injuries that made immediate surgery inadvisable.