Publications by authors named "Jose Antonio Merida-Cerro"
Certain DNA sequences can adopt a non-B form in the genome that interfere with DNA-templated processes, including transcription. Among the sequences that are intrinsically difficult to transcribe are those that tend to form R-loops, three-stranded nucleic acid structures formed by a DNA-RNA hybrid and the displaced ssDNA. Here we compared the transcription of an endogenous gene with and without an R-loop-forming sequence inserted.
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- DNA double-strand breaks (DSBs) are critical DNA damage events that threaten cell survival and genome stability, and their repair can vary based on the location of the breaks.
- Research indicates that while DNA-RNA hybrids can form at DSBs, these hybrids may actually hinder the recombinational repair process rather than aid it, particularly when breaks occur in transcribed regions.
- The study demonstrates that unresolved DNA-RNA hybrids at DSBs significantly disrupt the repair mechanism, emphasizing the need to eliminate these hybrids for effective recovery from DNA damage.