Volatile biomarkers of Gram-positive bacteria of clinical relevance as a tool for infection diagnosis.

Aim: Volatile organic compounds (VOCs) are being studied as potential biomarkers in many infections. Therefore, this study aimed to analyze the volatile profile of three Gram-positive bacteria of clinical relevance to identify potential volatile biomarkers that allow their differentiation.

Methods And Results: L.

Clinical and Ecological Impact of an Educational Program to Optimize Antibiotic Treatments in Nursing Homes (PROA-SENIOR): A Cluster, Randomized, Controlled Trial and Interrupted Time-Series Analysis.

Background: Antimicrobial stewardship programs (ASPs) are recommended in nursing homes (NHs), although data are limited. We aimed to determine the clinical and ecological impact of an ASP for NHs.

Methods: We performed a cluster, randomized, controlled trial and a before-after study with interrupted time-series analyses in 14 NHs for 30 consecutive months from July 2018 to December 2020 in Andalusia, Spain.

Impact of the COVID-19 Pandemic on Survival in the Patients With the Intra-Abdominal Infections.

To analyze the availability and access to the hospital for the patients with intra-abdominal infections (IAIs) by as a result of the coronavirus disease 2019 (COVID-19) pandemic and the impact of these changes in the diagnosis and their effects on the death of these patients. Two prospective observational cohorts of the patients with IAI by were conducted in 2016 (the pre-COVID-19, = 108) and in 2020 (during the COVID-19, = 96) at the University Hospital of Seville, Spain. The demographic and clinical variables of the patients were collected and analyzed.

Linezolid for therapy of meningitis: a cohort study of 26 patients.

Background: Linezolid has good penetration to the meninges and could be an alternative for treatment of meningitis. We assessed the efficacy and safety of linezolid therapy for this infection.

Methods: Retrospective multicenter cohort study of 26 adults treated with linezolid, derived from a cohort of 350 cases of meningitis diagnosed at 11 university hospitals in Spain (1981-2015).

A prospective, multicenter case control study of risk factors for acquisition and mortality in Enterobacter species bacteremia.

Background: Enterobacter is among the main etiologies of hospital-acquired infections. This study aims to identify the risk factors of acquisition and attributable mortality of Enterobacter bacteremia.

Methods: Observational, case-control study for risk factors and prospective cohort for outcomes of consecutive cases with Enterobacter bacteremia.

Epidemiological and clinical characteristics of Streptococcus tigurinus endocarditis.

Background: Streptococcus tigurinus was recently described as a new streptococcal species within the viridans group streptococci (VGS). The objectives of the present work were to analyse the clinical and microbiological characteristics of S. tigurinus isolated from patients with bacteraemias, to determine the prevalence of S.

[The relevance of correct identification and interpretation of susceptibility testing of Aeromonas spp. bacteremia isolates].

Objective: To assess the relevance of correct identification and interpretation of susceptibility testing of Aeromonas spp. bacteremia isolates using newly developed molecular methods in comparison to previous conventional methods.

Material And Methods: The study included 22 patients with bacteremia due to Aeromonas hydrophila group, microbiologically characterized using the MicroScan system.

[Utility of molecular biology techniques in the diagnosis of sexually transmitted diseases and genital infections].

Historically, the diagnosis of sexually transmitted diseases (STDs) has been difficult. The introduction of molecular biology techniques in microbiological diagnosis and their application to non-invasive samples has produced significant advances in the diagnosis of these diseases. Overall, detection of Neisseria gonorrhoeae by molecular biology techniques provides a presumptive diagnosis and requires confirmation by culture in areas with a low prevalence.

[Update on the epidemiology, diagnosis and treatment of sexually-transmitted infections].

In the last decade, cases of sexually-transmitted infections (STIs) have progressively increased in Europe. The reasons for this increase are unclear, but may involve changes in social behavior, migration and international travel, coupled with the emergence of risk groups that have not been taken into sufficient consideration to date. The routine use of molecular diagnostic techniques for many of these infections has solved many problems of sensitivity and the suitability of samples for microbiological diagnosis: non-invasive samples can be used, which has undoubtedly contributed to the increase in the number of cases.