Intraspecific variation of thermal tolerance along elevational gradients: the case of a widespread diving beetle (Coleoptera: Dytiscidae).

Species distributed across wide elevational gradients are likely to experience local thermal adaptation and exhibit high thermal plasticity, as these gradients are characterised by steep environmental changes over short geographic distances (i.e., strong selection differentials).

Genomic, morphological and physiological data support fast ecotypic differentiation and incipient speciation in an alpine diving beetle.

An intricate interplay between evolutionary and demographic processes has frequently resulted in complex patterns of genetic and phenotypic diversity in alpine lineages, posing serious challenges to species delimitation and biodiversity conservation planning. Here we integrate genomic data, geometric morphometric analyses and thermal tolerance experiments to explore the role of Pleistocene climatic changes and adaptation to alpine environments on patterns of genomic and phenotypic variation in diving beetles from the taxonomically complex Agabus bipustulatus species group. Genetic structure and phylogenomic analyses revealed the presence of three geographically cohesive lineages, two representing trans-Palearctic and Iberian populations of the elevation-generalist A.

Evolution of cold tolerance and thermal plasticity in life history, behaviour and physiology during a poleward range expansion.

Many species that are moving polewards encounter novel thermal regimes to which they have to adapt. Therefore, rapid evolution of thermal tolerance and of thermal plasticity in fitness-related traits in edge populations can be crucial for the success and speed of range expansions. We tested for adaptation in cold tolerance and in life history, behavioural and physiological traits and their thermal plasticity during a poleward range expansion.

Thermal evolution of life history and heat tolerance during range expansions toward warmer and cooler regions.

Species' range edges are expanding to both warmer and cooler regions. Yet, no studies directly compared the changes in range-limiting traits within the same species during both types of range expansions. To increase our mechanistic understanding of range expansions, it is crucial to disentangle the contributions of plastic and genetic changes in these traits.

Effects of salinity changes on aquatic organisms in a multiple stressor context.

Under global change, the ion concentration of aquatic ecosystems is changing worldwide. Many freshwater ecosystems are being salinized by anthropogenic salt inputs, whereas many naturally saline ones are being diluted by agricultural drainages. This occurs concomitantly with changes in other stressors, which can result in additive, antagonistic or synergistic effects on organisms.

Insect communities in saline waters consist of realized but not fundamental niche specialists.

Considering how organisms adapt to stress is essential if we are to anticipate biological responses to global change in ecosystems. Communities in stressful environments can potentially be assembled by specialists (i.e.

Validated HPLC-UV detection method for the simultaneous determination of ceftolozane and tazobactam in human plasma.

Aim: A simple, rapid, economical and sensitive HPLC-UV method was developed for the simultaneous quantification of ceftolozane and tazobactam in plasma samples.

Methodology: After deproteinization followed by a liquid-liquid back-extraction, the compounds were separated on a C18 column (150 mm × 4.6 mm, 5 μm) with UV-visible detection at 220 nm.

A new species of Aphelocheirus (Hemiptera: Heteroptera: Aphelocheiridae) from Morocco.

This paper provides the description of a new species of Aphelocheirus (Hemiptera: Heteroptera: Aphelocheiridae), Aphelocheirus pemae sp. nov. from Morocco.

Setting individualized positive end-expiratory pressure level with a positive end-expiratory pressure decrement trial after a recruitment maneuver improves oxygenation and lung mechanics during one-lung ventilation.

Background: We investigated whether individualized positive end-expiratory pressure (PEEP) improves oxygenation, ventilation, and lung mechanics during one-lung ventilation compared with standardized PEEP.

Methods: Thirty patients undergoing thoracic surgery were randomly allocated to the study or control group. Both groups received an alveolar recruitment maneuver at the beginning and end of one-lung ventilation.

How far could the alien boatman Trichocorixa verticalis verticalis spread? Worldwide estimation of its current and future potential distribution.

Invasions of alien species are considered among the least reversible human impacts, with diversified effects on aquatic ecosystems. Since prevention is the most cost-effective way to avoid biodiversity loss and ecosystem problems, one challenge in ecological research is to understand the limits of the fundamental niche of the species in order to estimate how far invasive species could spread. Trichocorixa verticalis verticalis (Tvv) is a corixid (Hemiptera) originally distributed in North America, but cited as an alien species in three continents.

[Echinocandins in a critically ill patient during continuous venovenous renal replacement].

Background: Critically ill patients with invasive candidiasis (IC) often suffer renal failure, which sometimes requires continuous renal replacement techniques (CRRT). Echinocandins are the first line treatment for IC in critically ill patients with mild or severe illness. Their elimination during CRRT should be negligible due to their pharmacokinetic and pharmacodynamic (PK/PD) profile, and dose adjustment are not needed, as suggested by the few reported clinical studies.

Virological and immunological features of active cytomegalovirus infection in nonimmunosuppressed patients in a surgical and trauma intensive care unit.

Cytomegalovirus (CMV) reactivation occurs frequently in critically ill patients. The natural course of CMV infection and the interaction between CMV and the adaptive immune system in this setting remain poorly defined. Fifty-three CMV-seropositive patients in a surgical and trauma intensive care unit were included in this study.

Cryopreserved and frozen hyaline cartilage imaged by environmental scanning electron microscope. An experimental and prospective study.

Objective: To obtain images of the articular surface of osteochondral grafts (fresh, frozen, and cryopreserved in RPMI) using an environmental scanning electron microscope (ESEM). To evaluate and compare the main morphological aspects of the chondral surface of the fresh, frozen, and cryopreserved grafts as visualized via ESEM.

Methods: The study was based on osteochondral fragments from the internal condyle of the knee joint of New Zealand rabbits, corresponding to the chondral surface from fresh, frozen, and cryopreserved samples.