Publications by authors named "Josan Marquez"
Article Synopsis
- Eukaryotic serpins are known protease inhibitors involved in various physiological processes, particularly in inflammatory bowel diseases, but bacterial serpins, especially those from gut microbiota, are less studied.
- Research focused on two putative serpin genes from the gut bacterium Eubacterium sireaum, leading to the discovery and purification of their proteins, named Siropins, which effectively inhibit specific human serine proteases related to inflammation.
- Biochemical analysis showed Siropins have distinct properties; Siropin 1 is stable at low pH while Siropin 2 is thermoactive, both exhibiting strong inhibitory capabilities that may help regulate intestinal protease activity.
Mismatch-repair factors have a prominent role in surveying eukaryotic DNA-replication fidelity and in ensuring correct meiotic recombination. These functions depend on MutL-homolog heterodimers with Mlh1. In humans, MLH1 mutations underlie half of hereditary nonpolyposis colorectal cancers (HNPCCs).
View Article and Find Full Text PDFRapid vitrification followed by the replacement of the vitrified water by a solvent (freeze substitution) and then resin is a widely used procedure for preparing biological samples for electron microscopy. The resulting plastic-embedded samples permit convenient room-temperature sectioning (microtomy) and can yield well preserved cellular structures. Here this procedure has been applied to crystalline protein samples, and it is shown that it is possible to freeze-substitute vitrified crystals while preserving some of their original diffraction properties.
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