Expression and functional analysis of the hydrogen peroxide biosensors HyPer and HyPer2 in C2C12 myoblasts/myotubes and single skeletal muscle fibres.

Hydrogen peroxide (HO) is generated in cells and plays an important role as a signalling molecule. It has been reported that HO is involved in physiological and pathological processes in skeletal muscle. However, HO detection in cells with traditional techniques produces frequent artefacts.