LBDNet interlaboratory comparison for the dicentric chromosome assay by digitized image analysis applying weighted robust statistical methods.

Purpose: This interlaboratory comparison was conducted to evaluate the performance of the Latin-American Biodosimetry Network (LBDNet) in analyzing digitized images for scoring dicentric chromosomes from irradiated blood samples. The exercise also assessed the use of weighted robust algorithms to compensate the uneven expertise among the participating laboratories.

Methods: Three sets of coded images obtained through the dicentric chromosome assay from blood samples irradiated at 1.

Assessment methods for inter-laboratory comparisons of the dicentric assay.

Purpose: To test the performance of different algorithms that can be used in inter-laboratory comparisons based on dicentric chromosome analysis, and to evaluate the impact of considering values different to calculate individual laboratory performance based on the ionizing radiation dose estimation.

Methods: Mean and standard deviation estimations in inter-laboratory comparisons are tested on simulated data and data from previously published inter-laboratory comparisons using three robust algorithms, Algorithm A, Algorithm B and Q/Hampel, all programmed in R-project language and implemented in a Shiny application. The simulated data were generated assuming three different probabilities to contaminate inter-laboratory comparisons samples with atypical dose values.

The BioDoseNet image repository used as a training tool for the dicentric assay.

This paper describes how the BioDoseNet image repository was used as a training tool for the dicentric assay. The training was implemented in three phases: introduction to dicentric scoring, dose response curve elaboration and dose assessment exercise. Four labs without previous experience in the dicentric assay participated and four modules of the repository were used.

Dose response of prematurely condensed chromosome rings after gamma irradiation.

Purpose: This paper presents a calibration curve for prematurely condensed chromosome rings (PCC-ring) which are scarce in cytogenetic dosimetry; here we analyzed the behavior of the PCC-ring frequency and PCC index after gamma irradiation in the dose interval from 0 to 20 Gy.

Materials And Methods: PCC-rings were induced in lymphocytes with Calyculin A. A total of 5788 PCC cells in G1, G2/M, and M/A stages were analyzed.