5-HT4 and 5-HT2 receptors antagonistically influence gap junctional coupling between rat auricular myocytes.

5-hydroxytryptamine-4 (5-HT(4)) receptors have been proposed to contribute to the generation of atrial fibrillation in human atrial myocytes, but it is unclear if these receptors are present in the hearts of small laboratory animals (e.g. rat).

Caffeine-induced immobilization of gating charges in isolated guinea-pig ventricular heart cells.

The effects of 10 mM caffeine (CAF) on intramembrane charge movements (ICM) were studied in isolated guinea-pig ventricular heart cells with the whole-cell patch-clamp technique. In the presence of CAF, the properties (voltage dependence, maximum Q(ON) [Q(max)], availability with voltage) of Q(ON) charge activated from -110 mV were barely affected. Following a 100 ms prepulse to -50 mV to decrease the participation of charges originating from Na channels, the voltage dependence of Q(ON) was shifted by 5 mV (negative component) and by 10 mV (positive component) towards negative potentials, and Q(max) was depressed by 16.