Publications by authors named "Jordane Bousquet"
The identification of specific protein markers for breast cancer would provide the basis for early diagnosis. Particularly, membrane and membrane-associated proteins are rich in targets for antibodies that may constitute suitable biomarkers of carcinogenesis. However, membrane proteins separation using 2-DE remains difficult.
View Article and Find Full Text PDFThis study presents an improved method for the complete transfer of proteins separated by two-dimensional gel electrophoresis to a membrane, specifically designed for the screening and identification of antigens recognized by autoantibodies in patients with breast cancer (BCP) and healthy volunteers. This paper reports the evaluation of this technique using proteins from MCF7 as a source of antigens following 2-DE separation. The appropriate quantity of protein to be loaded on gels (150 microg) has been determined, the aim being a complete and reproducible recovery of all separated proteins onto the polyvinylidene fluoride membrane (2D-blot) after a semi-dry electrotransfer.
View Article and Find Full Text PDFArticle Synopsis
- The integration of robots significantly enhances the efficiency and data quality of proteomic workflows, particularly in high-throughput projects.
- Automation of post-two-dimensional gel electrophoresis steps, especially in peptide mass fingerprinting (PMF), is critical for fluid workflow.
- A newly developed sandwich method for preparing samples for MALDI mass spectrometry showed superior success rates and protein sequence coverage compared to traditional methods.