A specific radio-immunological assay for prednisolone in plasma.

A new, specific radio-immunological assay (R.I.A.

Objective determinations of sensibility in the upper extremity. Part III. Application of cutaneous stimuli in patients with peripheral nerve lesions.

Quantitative values for pressure, electrical, and two-point touch stimulation were examined in five patients with peripheral nerve injuries of the ulnar or median nerves. Analyses of profiles generated for each stimulus application in each patient participating in this preliminary study suggest that, following the reading of a sensitization statement, pressure and electrical shock threshold values are clearly reduced compared to similar measurements in the unaffected limb. Values obtained prior to the statement indicated progressively reduced thresholds, however.

Objective determinations of sensibility in the upper extremity. Part II. Application of cutaneous stimuli in control subjects.

Sensitization statements were read to two groups of normal subjects to determine whether quantifiable thresholds for pressure, electrical, or tactile stimuli could be altered. Significant reductions in perception thresholds were observed for pressure and digital pulsed stimulation, but not for stimulation to the discomfort threshold, or two point discrimination. These data seem to suggest that increased sensitization is related to the ease of stimulus applications and the comparative simplicity of sensory processing.

Objective determinations of sensibility in the upper extremity. Part I. Median nerve sensory conduction velocities.

Distal sensory conduction velocities were determined in 109 normal median nerves. A significant decrease in sensory conduction velocity was noted with increasing age. Latencies increased with age but this relationship was not significant in men.

Quantitation of digoxigenin in serum following oral administration of digoxin in humans.

Separation of the acid breakdown products from digoxin in serum was accomplished by high performance liquid chromatography (HPLC). The major, less cardioactive, product digoxigenin was quantitated by several different commercial antisera from digoxin radioimmunoassay (RIA) kits. When two normal subjects were given digoxigenin (0.

Analysis of prednisolone in plasma by high-performance liquid chromatography.

A sensitive, specific high-performance liquid chromatographic procedure for the determination of prednisolone in plasma is described. The organic solvent extract from plasma is chromatographed on a silica gel column using a mobile phase of 0.2% glacial acetic acid, 6% ethanol, 30% methylene chloride in n-hexane on a high-performance liquid chromatograph fitted with an ultraviolet dector (254 nm).