Changes in ultrasonic properties of liver tissue in vitro during heating-cooling cycle concomitant with thermal coagulation.

The present work considers the ultrasonic properties of porcine liver tissue in vitro measured during heating concomitant with thermal coagulation followed by natural cooling, so as to provide information about changes in the ultrasonic properties of the tissue after thermal coagulation. The excised liver samples were heated in a degassed water bath up to 75°C and naturally cooled down to 30°C. The tissue was observed to begin thermally coagulating at temperatures lower than 75°C.

Immunohistochemical localization of heat shock protein 27 in the retina of pigs.

The expression of heat shock protein 27 (HSP27) was examined in the retinas of pigs. Western blot analysis detected the expression of HSP27 in the retinas of 1-day-old piglets and showed that it was enhanced in the retinas of 6-month-old adult pigs. Immunohistochemically, HSP27 immunostaining was seen mainly in ganglion cell bodies in the ganglion cell layer, and in some processes of astrocytes in the innermost nerve fiber layer.

Temporal expression of osteopontin and CD44 in rat brains with experimental cryolesions.

Expression of osteopontin and CD44 in the brain was studied after cryolesioning to understand how osteopontin and its receptor, CD44, are involved in processes in the brains of rats with cryolesions. Western blot analysis showed that osteopontin increased significantly at days 4 and 7 post-injury and declined slightly thereafter in cryolesioned brains in comparison with levels in sham-operated controls. An immunohistochemical study localized osteopontin in activated microglia/macrophages in the core lesions, where the majority of macrophages proliferate.

Electroacupuncture and moxibustion for correction of abomasal displacement in dairy cattle.

This study was performed to investigate the therapeutic effect of electroacupuncture and moxibustion on abomasal displacement in dairy cattle. After acupuncture needles were inserted bilaterally into the acupoints, 'Pi yu', 'Wei yu' and 'Guan yuan yu' , electronic stimulation (5 Hz and 10 V, 20 minutes) was conducted once a day for 3 days consecutively. Modified moxa patch was also applied at the same acupoints as in acupuncture for 3 days consecutively.