Safety Assessment of Camelid-Derived Single-Domain Antibody as Feed Additive for Juvenile Whiteleg Shrimp () Against White Spot Syndrome Virus.

A six-week feeding trial was conducted to assess the safety of single-domain antibodies (sdAbs) derived from camelids against the white spot syndrome virus (WSSV) (WSSVvp28 was used as the antigen), focusing on the whole-organism responses and molecular-level changes in juvenile whiteleg shrimp (). Five experimental diets with varying levels of sdAbs were formulated: CON (no sdAb supplementation); SDA (8.20% of sdAbs); SDA (16.

Effect of Endurance Exercise Training on Gut Microbiota and ER Stress.

Regular exercise as part of one's lifestyle is well-recognized for its beneficial effect on several diseases such as cardiovascular disease and obesity; however, many questions remain unanswered regarding the effects of exercise on the gut environment. This study aimed to investigate the impact of long-term endurance exercise on modulating inflammation and endoplasmic reticulum (ER) stress. Fifteen-week-old male Sprague-Dawley (SD) rats were subjected to six months of endurance treadmill training, while age-matched controls remained sedentary.

Optimization of turbidity removal and floc formation for ballasted flocculation using magnetite-based agents by response surface methodology.

In this study, a static mixer was used as an alternative to the existing flash mixing method for ballasted flocculation to assess the turbidity removal and ballasted floc formation characteristics. Synthetic magnetite exhibits excellent properties, such as high specific gravity, hydrophobicity, and wear resistance, making it a suitable ballast agent (BA). The experimental design was optimized using the response surface methodology.

Complete Mitochondrial Genome and Its Phylogenetic Position in Red Algae from South Korea.

The mitogenome is an important tool in taxonomic and evolutionary studies. Only a few complete mitogenomes have been reported for red algae. Herein, we reported the complete mitochondrial genome sequence of (Harvey) Filloramo, G.

Adiponectin restores the obesity-induced impaired immunomodulatory function of mesenchymal stromal cells glycolytic reprogramming.

Obesity has been known to negatively modulate the life-span and immunosuppressive potential of mesenchymal stromal cells (MSC). However, it remains unclear what drives the compromised potency of obese MSC. In this study, we examined the involvement of adiponectin, an adipose tissue-derived hormone, in obesity-induced impaired therapeutic function of MSC.

Optimization of KCO exposure conditions using response surface methodology for CO capture with 2-methylpiperazine and monoethanolamine as promoters.

In this study, the optimization of potassium carbonate (KCO) exposure conditions for CO capture with the use of 2-methypiperazine (2MPz) and monoethanolamine (MEA) as promoters was investigated. The tested operating conditions for the CO capture process included the pH, temperature, KCO dose, gas flow rate, and pressure, and their effect on the CO absorption/desorption rate and CO absorption efficiency was assessed. Response surface methodology (RSM) was also employed to determine the equations for the optimal long-term operating conditions.

Efficacy of White Spot Syndrome Virus Protein VP28-Expressing as an Oral Vaccine for Shrimp.

The white spot syndrome virus (WSSV) is the causative agent of white spot disease, which kills shrimp within a few days of infection. Although WSSV has a mortality rate of almost 100% and poses a serious threat to the shrimp farming industry, strategies for its prevention and treatment are extremely limited. In this study, we examined the efficacy of VP28, a recombinant WSSV protein expressed in (), as an oral shrimp vaccine.

Regulatory effects of potassium channel blockers on potassium channel genes upon nervous necrosis virus infection in sevenband grouper Hyporthodus septumfasciatus.

Ion channels in fishes regulate the flow of important ions that play an active role in the excitation and transmission of impulses through neuronal cells. Specific housekeeping genes translates into proteins and selectively permeabilize and facilitate ion crossover transmissions. Potassium (K) channels play a crucial role in a wide range of functions such as cell volume regulation, hormone secretion, synaptic transmission and muscle contraction.

Role of inorganic foulants in the aging and deterioration of low-pressure membranes during the chemical cleaning process in surface water treatment: A review.

Low-pressure membrane (LPM) filtration, including microfiltration (MF) and ultrafiltration (UF), is a promising technology for the treatment of surface water for drinking and other purposes. Various configurations and operational sequences have been developed to ensure the sustainable provision of clean water by overcoming fouling problems. In the literature, various periodic physical and/or chemical approaches to the cleaning of LPMs have been reported, but little data is available on the aging of MF/UF membranes that results from the interaction between the foulants and the cleaning agent.

Complete Mitogenome Sequencing, Annotation, and Phylogeny of , a Red Alga with Intronic Gene.

The mitochondrial genome (mitogenome) is essential for identifying species and tracing genetic variation, gene patterns, and evolutionary studies. Here, the mitogenome of was sequenced on the Illumina sequencing platform. This circular mitogenome (28,265 bp) contains 49 genes, including three rRNAs, twenty transfer RNAs (tRNAs), and twenty-six protein-coding genes (PCGs).

Field experiments on chemical and biological changes of thin-layer oyster shells capping sediments in dense aquaculture area.

Thin-layer oyster shell capping has been proposed as a method for improving contaminated coastal environments. Field experiments were conducted to investigate the effects of oyster shell capping on nutrient concentrations, microorganisms, and macrobenthic communities. The concentration of PO-Pin the experimental area decreased by approximately 38% more than in the control, due to phosphorus fixation of oyster shells and the presence of Proteobacteria.

Structural and performance variation of PES/PVDF membranes after exposure to the pretreated feed water of CECs.

In this study, the removal efficiency of chemicals of emerging concerns (CECs) was evaluated under exposure to various doses of UV/HO-based oxidation processes in combination with membrane filtration for three cleaning cycles. Polyethersulphone (PES) and polyvinylidene fluoride (PVDF) materials based membranes were used for this study. The chemical cleaning of the membranes was performed by immersion of the membranes into 1 N HCl followed by adding 3000 mg.

Natural Variations in the Benthic Environment and Bacterial Communities of Coastal Sediments around Aquaculture Farms in South Korea.

Unlabelled: The aim of this study was to examine the possible seasonal variations in the nutrients (dissolved inorganic nitrogen-DIN and phosphorus) and benthic bacterial communities in marine aquaculture surrounding sediments. The study areas were Geoje, Tongyeong, and Changwon bays in Korea, which are famous for oysters (), , and warty sea squirt () farming, respectively. The study sites included semi-enclosed coastal areas with a low seawater exchange rate.

Methionine consumption by cancer cells drives a progressive upregulation of PD-1 expression in CD4 T cells.

Programmed cell death protein 1 (PD-1), expressed on tumor-infiltrating T cells, is a T cell exhaustion marker. The mechanisms underlying PD-1 upregulation in CD4 T cells remain unknown. Here we develop nutrient-deprived media and a conditional knockout female mouse model to study the mechanism underlying PD-1 upregulation.

Analysis of the complete mitochondrial genome sequence of Japanese butterflyfish, (Chaetodontiformes, Chaetodontidae).

Japanese butterflyfish () belong to the family Chaetodontidae and order Chaetodontiformes. It has circular mitochondrial genome of 16,507 bp in length with 55.4% of A + T content and has 37 genes, including 22 tRNA, 2 rRNA, and 13 protein-coding genes, in addition to a control region.

The complete sequence of the mitochondrial DNA and phylogenetic analysis of the marine red alga (Rhodophyta: Halymeniales).

(Holmes, 1896) is a red alga belonging to the order Halymeniales and phylum Rhodophyta. In this study, the complete mitochondrial DNA (mtDNA) of has been described. The complete circular mtDNA of was 28,503 bp in length, with an A + T content of 68.

The effectiveness and adsorption mechanism of iron-carbon nanotube composites for removing phosphate from aqueous environments.

This study successfully employed iron-carbon nanotubes (Fe-CNT) to recover phosphate (P) from water. We examined the effects of various iron concentrations denoted by Fe-CNT-1 and Fe-CNT-2 on P removal and compared them with pristine carbon nanotubes (CNTs). The adsorption capacity of Fe-CNTs was much better than pristine CNTs.

Complete mitochondrial genome of brown-banded butterflyfish (Chaetodontiformes, Chaetodontidae) and phylogenetic analysis.

The complete mitochondrial genome of the (Temminck and Schlegel, 1844) was first determined in this study, which is 16,490 bp in length, containing 13 protein-coding genes, two rRNA genes, and 22 tRNA. Out of 37 mitochondrial genes, except for and eight tRNA (, , , , , , , ) genes were encoded on the L-strand, the others were encoded on the H-strand. The overall base composition includes A (28.

The complete mitochondrial genome of threadfin butterflyfish, (Chaetodontiformes: Chaetodontidae) and phylogenetic analysis.

(Forsskal, 1775) belongs to the family Chaetodontidae and the order Chaetodontiformes. Here, we report the complete mitochondrial genome of assembled using the Illumina MiSeq platform. The circular mitochondrial genome of is 16,527 bp long, has an A + T content of 54.

Temperature dependent cellular, and epigenetic regulatory mechanisms underlying the antiviral immunity in sevenband grouper to nervous necrosis virus infection.

Changes in the thermal optima of fish impacts changes in the physiology and immune response associated with infections. The present study showed that at suboptimal temperatures (17 °C), the host tries to evade viral infection by downregulating the inflammatory response through enhanced neuronal protection. There was significantly less abundance of IgM + B cells in the 17 °C group compared to that in the 25 °C group.

Mitigation of biofouling with co-deposition of polydopamine and curcumin on the surface of a thin-film composite membrane.

Reverse osmosis (RO) membrane has been widely used in various water treatment fields as an efficient desalination technology, but serious biofouling problem arises in the actual application process. Curcumin is known as a natural compound that can reduce biofouling by inhibiting the growth of microorganisms based on quorum sensing. Dopamine, a molecule with excellent adhesion and functionalization on the material's surface, has high research value for applying a curcumin coating to the membrane surface.

Complete mitochondrial genome of scorpionfish (Actinopterygii).

(Temminck and Schlegel, 1843) is a marine fish, in the family Scorpaenidae, order Scorpaeniformes, class Actinopterygii of the phylum Chordata. The first species of with a complete mitochondrial genome is described in the present study. The circular mitochondrial genome of has 17,202 bp with 54.

Complete mitochondrial genome and phylogenetic analysis of the marine red alga (Rhodophyta: Halymeniales).

((Harvey) Kawaguchi & Wang, 2002) is a red alga in the order Halymeniales of the phylum Rhodophyta. The entire mitogenome of was sequenced and compared to related Halymeniales species. The entire circular-mitogenome is 25,988 bp long, has 27.

NLRC3 attenuates antiviral immunity and activates inflammasome responses in primary grouper brain cells following nervous necrosis virus infection.

NLRC3 is identified as a unique regulatory NLR involved in the modulation of cellular processes and inflammatory responses. In this study, a novel Nod like receptor C3 (NLRC3) was functionally characterized from seven band grouper in the context of nervous necrosis virus infection. The grouper NLRC3 is highly conserved and homologous with other vertebrate proteins with a NACHT domain and a C-terminal leucine-rich repeat (LRR) domain and an N-terminal CARD domain.