Publications by authors named "Jonghyen Kim"
Article Synopsis
- - Enteroviruses (EVs) are the main cause of aseptic meningitis globally, and detecting their RNA in clinical samples can enhance patient care, especially for infants and young kids.
- - A study compared the GeneXpert Enterovirus Assay (GXEA) and real-time one step RT-PCR (RTo-PCR) for routine enterovirus diagnosis, finding GXEA had a 100% sensitivity and specificity, while RTo-PCR had a sensitivity of 65%.
- - Both assays are effective for detecting enteroviruses in clinical samples, indicating that nucleic acid amplification methods are valuable for diagnosing enteroviral infections.
Background: Enterovirus (EV) infections are commonly associated with encephalitis and meningitis. Detection of enteroviral RNA in clinical specimens has been demonstrated to improve the management of patients, by ruling out other causes of disease.
Method: To develop a sensitive and reliable assay for routine laboratory diagnosis, we developed a real-time one step reverse transcription polymerase chain reaction (RT-PCR) assay with minor groove binder probes and primers modified with complementary locked primer technology (TMC-PCR).
To increase detection sensitivity, we modified primers using complementary locked primer (CLP) technology. The sensitivity of the reverse transcription-PCR (RT-PCR) with CLP-modified primers was 10- to 100-fold higher than that of RT-PCR without these primers. CLP-modified primers can increase sensitivity, providing a widely accessible method for molecular diagnosis.
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