Environmental analysis of returnable packaging systems in different eCommerce business and packaging management models.

There is growing environmental concern regarding the increasing quantity of packages in retail eCommerce. This study investigated the environmental impact of two returnable packaging formats, performing life cycle assessment (LCA) case studies based on the Canadian apparel eCommerce market. In case study 1, the brand owner sold and shipped its products to final consumers using an expendable mailer and a returnable mailer that was managed and supplied via the centralized model.

LongTR: genome-wide profiling of genetic variation at tandem repeats from long reads.

Tandem repeats are frequent across the human genome, and variation in repeat length has been linked to a variety of traits. Recent improvements in long read sequencing technologies have the potential to greatly improve tandem repeat analysis, especially for long or complex repeats. Here, we introduce LongTR, which accurately genotypes tandem repeats from high-fidelity long reads available from both PacBio and Oxford Nanopore Technologies.

Analysis and benchmarking of small and large genomic variants across tandem repeats.

Tandem repeats (TRs) are highly polymorphic in the human genome, have thousands of associated molecular traits and are linked to over 60 disease phenotypes. However, they are often excluded from at-scale studies because of challenges with variant calling and representation, as well as a lack of a genome-wide standard. Here, to promote the development of TR methods, we created a catalog of TR regions and explored TR properties across 86 haplotype-resolved long-read human assemblies.

Genome-wide profiling of genetic variation at tandem repeat from long reads.

Tandem repeats are frequent across the human genome, and variation in repeat length has been linked to a variety of traits. Recent improvements in long read sequencing technologies have the potential to greatly improve TR analysis, especially for long or complex repeats. Here we introduce LongTR, which accurately genotypes tandem repeats from high fidelity long reads available from both PacBio and Oxford Nanopore Technologies.

Benchmarking of small and large variants across tandem repeats.

Tandem repeats (TRs) are highly polymorphic in the human genome, have thousands of associated molecular traits, and are linked to over 60 disease phenotypes. However, their complexity often excludes them from at-scale studies due to challenges with variant calling, representation, and lack of a genome-wide standard. To promote TR methods development, we create a comprehensive catalog of TR regions and explore its properties across 86 samples.

A deep population reference panel of tandem repeat variation.

Tandem repeats (TRs) represent one of the largest sources of genetic variation in humans and are implicated in a range of phenotypes. Here we present a deep characterization of TR variation based on high coverage whole genome sequencing from 3550 diverse individuals from the 1000 Genomes Project and H3Africa cohorts. We develop a method, EnsembleTR, to integrate genotypes from four separate methods resulting in high-quality genotypes at more than 1.

Continuous cuffless blood pressure monitoring using photoplethysmography-based PPG2BP-net for high intrasubject blood pressure variations.

Continuous, comfortable, convenient (C3), and accurate blood pressure (BP) measurement and monitoring are needed for early diagnosis of various cardiovascular diseases. To supplement the limited C3 BP measurement of existing cuff-based BP technologies, though they may achieve reliable accuracy, cuffless BP measurement technologies, such as pulse transit/arrival time, pulse wave analysis, and image processing, have been studied to obtain C3 BP measurement. One of the recent cuffless BP measurement technologies, innovative machine-learning and artificial intelligence-based technologies that can estimate BP by extracting BP-related features from photoplethysmography (PPG)-based waveforms have attracted interdisciplinary attention of the medical and computer scientists owing to their handiness and effectiveness for both C3 and accurate, i.

TRviz: a Python library for decomposing and visualizing tandem repeat sequences.

Summary: TRviz is an open-source Python library for decomposing, encoding, aligning and visualizing tandem repeat (TR) sequences. TRviz takes a collection of alleles (TR containing sequences) and one or more motifs as input and generates a plot showing the motif composition of the TR sequences.

Availability And Implementation: TRviz is an open-source Python library and freely available at https://github.

A deep population reference panel of tandem repeat variation.

Tandem repeats (TRs) represent one of the largest sources of genetic variation in humans and are implicated in a range of phenotypes. Here we present a deep characterization of TR variation based on high coverage whole genome sequencing from 3,550 diverse individuals from the 1000 Genomes Project and H3Africa cohorts. We develop a method, EnsembleTR, to integrate genotypes from four separate methods resulting in high-quality genotypes at more than 1.

Variable number tandem repeats (VNTRs) as modifiers of breast cancer risk in carriers of BRCA1 185delAG.

Despite substantial efforts in identifying both rare and common variants affecting disease risk, in the majority of diseases, a large proportion of unexplained genetic risk remains. We propose that variable number tandem repeats (VNTRs) may explain a proportion of the missing genetic risk. Herein, in a pilot study with a retrospective cohort design, we tested whether VNTRs are causal modifiers of breast cancer risk in 347 female carriers of the BRCA1 185delAG pathogenic variant, an important group given their high risk of developing breast cancer.

Prevalence of hereditary tubulointerstitial kidney diseases in the German Chronic Kidney Disease study.

Hereditary chronic kidney disease (CKD) appears to be more frequent than the clinical perception. Exome sequencing (ES) studies in CKD cohorts could identify pathogenic variants in ~10% of individuals. Tubulointerstitial kidney diseases, showing no typical clinical/histologic finding but tubulointerstitial fibrosis, are particularly difficult to diagnose.

Detecting tandem repeat variants in coding regions using code-adVNTR.

The human genome contains more than one million tandem repeats (TRs), DNA sequences containing multiple approximate copies of a motif repeated contiguously. TRs account for significant genetic variation, with 50 + diseases attributed to changes in motif number. A few diseases have been to be caused by small indels in variable number tandem repeats (VNTRs) including poly-cystic kidney disease type 1 (MCKD1) and monogenic type 1 diabetes.

Scalable Scheduling of Semiconductor Packaging Facilities Using Deep Reinforcement Learning.

Reinforcement learning (RL) has emerged as a promising approach for scheduling semiconductor operations. Yet, it is still challenging to solve large-scale scheduling problems based on an RL method since learning complexity grows fast as the size of shop floor increases. This challenge becomes more apparent when solving the scheduling problems with a diverse number of job types, which leads to the difficulties in exploration and function approximation in RL.

Investigation of the Effect of Pallet Top-Deck Stiffness on Corrugated Box Compression Strength as a Function of Multiple Unit Load Design Variables.

Unit loads consisting of a pallet, packages, and a product securement system are the dominant way of shipping products across the United States. The most common packaging types used in unit loads are corrugated boxes. Due to the great stresses created during unit load stacking, accurately predicting the compression strength of corrugated boxes is critical to preventing unit load failure.

High-rate biohydrogen production from xylose using a dynamic membrane bioreactor.

This study aimed a high-rate dark fermentative H production from xylose using a dynamic membrane module bioreactor (DMBR) with a 444-μm pore polyester mesh. 20 g xylose/L was fed continuously to the DMBR at different hydraulic retention times (HRTs) from 12 to 3 h at 37 °C. The maximum average H yield (HY) and H production rate (HPR) at 3 h HRT were found to be 1.

Effect of genus Clostridium abundance on mixed-culture fermentation converting food waste into biohydrogen.

This study examined the effect of various inocula on mixed-culture dark fermentative H production from food waste. Heat-treated and frozen H-producing granular sludge (HPG) grown with monomeric sugars showed a higher H yield, production rate, and acidogenic efficiency along with a shorter lag phase than heat-treated methanogenic sludge. Among three different methods of methanogenic sludge inoculation, inoculation after centrifugation showed better H production performance.

Production and characterization of crude laccase from Irpex sp. JS7 that decolorizes synthetic and natural melanin.

The JS7 strain, isolated from an old forest tree, produces extracellular enzymes that decolorize synthetic and natural melanin from human hair. Phylogenetic analysis based on the internal transcribed spacer (ITS) sequence indicated that JS7 belongs to the genus Irpex. The JS7 strain has laccase activity while it lacks manganese and lignin peroxidase activity, which suggests that the JS7 strain melanin decolorization activity originated from laccase.

Dynamic membrane bioreactor for high rate continuous biohydrogen production from algal biomass.

This study aimed to achieve continuous biohydrogen production from red algal biomass using a dynamic membrane bioreactor (DMBR). The DMBR was continuously fed with pretreated Echeuma spinosum containing 20 g/L hexose. The highest average hydrogen production rate (HPR) of 21.

A BCI Based Alerting System for Attention Recovery of UAV Operators.

As unmanned aerial vehicles have become popular, the number of accidents caused by an operator's inattention have increased. To prevent such accidents, the operator should maintain an attention status. However, limited research has been conducted on the brain-computer interface (BCI)-based system with an alerting module for the operator's attention recovery of unmanned aerial vehicles.

Variable number tandem repeats mediate the expression of proximal genes.

Variable number tandem repeats (VNTRs) account for significant genetic variation in many organisms. In humans, VNTRs have been implicated in both Mendelian and complex disorders, but are largely ignored by genomic pipelines due to the complexity of genotyping and the computational expense. We describe adVNTR-NN, a method that uses shallow neural networks to genotype a VNTR in 18 seconds on 55X whole genome data, while maintaining high accuracy.

High-rate mesophilic hydrogen production from food waste using hybrid immobilized microbiome.

This study examined the feasibility of dark fermentative biohydrogen production from food waste using hybrid immobilization in mesophilic condition. Among four different organic loading rates (OLRs), the highest average hydrogen production rate (HPR) of 9.82 ± 0.

State-of-the-art technologies for continuous high-rate biohydrogen production.

Dark fermentation is a technically feasible technology for achieving carbon dioxide-free hydrogen production. This review presents the current findings on continuous hydrogen production using dark fermentation. Several operational strategies and reactor configurations have been suggested.

Evaluation of genetic toxicity, acute and sub-chronic oral toxicity and systemic safety of and 50% ethanolic extract mixture (APRG64) in vitro and in vivo (rodent and non-rodent animal models).

(AP) and (RG) are traditional medicinal plants. The bioflavonoid composition standardized by HPLC analysis was named APRG64. Despite many studies reported to beneficial bioactivities of AP and RG, very limited range of toxicity tests have documented.

Enhancing anaerobic digestion for rural wastewater treatment with granular activated carbon (GAC) supplementation.

Notwithstanding many efforts to increase the efficiency of anaerobic digestion at low-temperature (winter) conditions, a cost-effective and efficient method is lacking. This study proposes a low-cost method of low-temperature (<35 °C) anaerobic digestion of wastewater, involving supplementation with granular activated carbon (GAC). Supplementation with GAC was found to reduce the lag time by 29.

Refolding, characterization, and dye decolorization ability of a highly thermostable laccase from Geobacillus sp. JS12.

A putative laccase gene (lacG) from Geobacillus sp. JS12 was cloned and expressed as a fusion protein with six histidine residues in Escherichia coli BL21 (DE3) cells, and the protein was primarily found in inclusion bodies. The resulting insoluble proteins were solubilized with 6 M guanidine HCl and refolded using an on-column refolding procedure.