A Multilayered Screening Method for the Identification of Regulatory Genes in Rice by Agronomic Traits.

We developed a multilayered screening method that integrates both genome and transcriptome data to effectively identify regulatory genes in rice (). We tested our method using eight rice accessions that differed in three important nutritional and agricultural traits, anthocyanin biosynthesis, amylose content, and heading date. In the genome resequencing of eight rice accessions with 24 RNA sequencing experiments, 98% of the preprocessed reads could be uniquely mapped to the reference genome, resulting in the identification of 42,699 unique transcripts.

Chromosomal cephalosporinase in Enterobacter hormaechei as an ancestor of ACT-1 plasmid-mediated AmpC β-lactamase.

In this study of the diversity of AmpC β-lactamase in clinical isolates of Enterobacter spp., a strain was found carrying the plasmid-mediated AmpC β-lactamase ACT-1 gene on its chromosome. The strain was identified as Enterobacter hormaechei using phylogenetic analysis of 16S rRNA and hsp60 genes.