Multimodal Spatial Profiling Reveals Immune Suppression and Microenvironment Remodeling in Fallopian Tube Precursors to High-Grade Serous Ovarian Carcinoma.

High-Grade Serous Ovarian Cancer (HGSOC) originates from fallopian tube (FT) precursors. However, the molecular changes that occur as precancerous lesions progress to HGSOC are not well understood. To address this, we integrated high-plex imaging and spatial transcriptomics to analyze human tissue samples at different stages of HGSOC development, including p53 signatures, serous tubal intraepithelial carcinomas (STIC), and invasive HGSOC.

Cobalt-Based ZIF Composite Membranes: In Situ Defect Engineering for Enhanced Water Stability and Gas Separation.

Porous coordination polymers with excellent molecular sieving ability, high dispersibility, and good compatibility with engineered polymer matrices hold promise for various industrial applications, such as gas separation and battery separators. Here, an in situ defect engineering approach is proposed for highly processable cobalt (Co)-based zeolitic imidazolate frameworks (ZIFs) with enhanced molecular sieving ability and water stability. By varying alkylamine (AA) modulators, the pore structures and textural properties of ZIFs can be fine-tuned.

Correlation between Hearing Impairment and the Triglyceride Glucose Index in Middle-Aged Female Based on a Korean National Health and Nutrition Examination Survey.

: This study aimed to investigate the association between insulin resistance, as measured by the triglyceride-glucose index (TyG index), and hearing impairment in middle-aged women in Korea. : This cross-sectional survey utilized data from the Korea National Health and Nutrition Examination Survey (KNHANES) IV (2007-2009), specifically from the period after July 21, 2009, when hearing test results became available, and from the KNHANES V (2010-2012). This study was conducted on 5416 women aged 40 to 69 who had completed both the health examination survey and audiometric tests, excluding those with missing data on menopausal status and the use of hormone replacement therapy.

Novel, cold-adapted D-laminaribiose- and D-glucose-releasing GH16 endo-β-1,3-glucanase from PAMC 29290, a psychrotolerant bacterium from Arctic marine sediment.

Endo-β-1,3-glucanase is a glycoside hydrolase (GH) that plays an essential role in the mineralization of β-glucan polysaccharides. In this study, the novel gene encoding an extracellular, non-modular GH16 endo-β-1,3-glucanase (GluH) from PAMC 29290 isolated from Arctic marine sediment was discovered through an analysis of its whole genome sequence and subsequently overexpressed in BL21. The 870-bp GluH gene encoded a protein featuring a single catalytic GH16 domain that shared over 61% sequence identity with uncharacterized endo-β-1,3-glucanases from diverse species, as recorded in the National Center for Biotechnology Information database.

The Effect of One-Year Fermentation of Fruit () Sugar Syrup on Amygdalin Level: A Natural Toxic Compound.

() is an economically important fruit in Korea. Recently, public interest in sugar syrup is increasing. However, the presence of toxic amygdalin in the fruit syrup is a concern.

Glycoside-metabolizing oxidoreductase D3dgpA from human gut bacterium.

The Gfo/Idh/MocA family enzyme DgpA was known to catalyze the regiospecific oxidation of puerarin to 3"-oxo-puerarin in the presence of 3-oxo-glucose. Here, we discovered that D3dgpA, cloned from the human gut bacterium sp. MRG-IFC3, catalyzed the regiospecific oxidation of various -/-glycosides, including puerarin, in the presence of methyl β-D-3-oxo-glucopyranoside.

Flavonoids Biotransformation by Human Gut Bacterium sp. MRG-IFC3 Cell-Free Extract.

Human gut bacterium sp. MRG-IFC3 is unique in that it is capable of metabolizing puerarin, an isoflavone -glycoside, whereas it shows broad substrate glycosidase activity for the various flavonoid -glycosides. To address the question on the substrate specificity, as well as biochemical characteristics, cell-free biotransformation of flavonoid glycosides was performed under various conditions.

STMND1 is a phylogenetically ancient stathmin which localizes to motile cilia and exhibits nuclear translocation that is inhibited when soluble tubulin concentration increases.

Stathmins are small, unstructured proteins that bind tubulin dimers and are implicated in several human diseases, but whose function remains unknown. We characterized a new stathmin, STMND1 (Stathmin Domain Containing 1) as the human representative of an ancient subfamily. STMND1 features a N-terminal myristoylated and palmitoylated motif which directs it to membranes and a tubulin-binding stathmin-like domain (SLD) that contains an internal nuclear localization signal.

Separation of High-Purity CH from Binary CH/CO Using Robust Al-Based MOFs Comprising Nitrogen-Containing Heterocyclic Dicarboxylate.

In this study, three nitrogen-containing aluminum-based metal-organic frameworks (Al-MOFs), namely, CAU-10pydc, MOF-303, and KMF-1, were investigated for the efficient separation of a CH/CO gas mixture. Among these three Al-MOFs, KMF-1 demonstrated the highest selectivity for CH/CO separation (6.31), primarily owing to its superior CH uptake (7.

Safety Analysis of Korean Cottage Industries' , a Traditional Fermented Soybean Product: A Special Reference to Biogenic Amines.

The typical Korean diet contains a significant quantity of owing to its unique taste and health benefits. However, the presence of anti-nutritional and toxic substances, such as biogenic amines and microbial pathogens, in has resulted in a loss of revenue and poor consumer health. The present study focused on the identification and quantification of different biogenic amines, pathogenic , and yeast counts in 36 products (designated as De-1 to De-36, 500 g each) procured from the different cottage industries situated in different parts of the Republic of Korea.

2D and 3D multiplexed subcellular profiling of nuclear instability in human cancer.

Nuclear atypia, including altered nuclear size, contour, and chromatin organization, is ubiquitous in cancer cells. Atypical primary nuclei and micronuclei can rupture during interphase; however, the frequency, causes, and consequences of nuclear rupture are unknown in most cancers. We demonstrate that nuclear envelope rupture is surprisingly common in many human cancers, particularly glioblastoma.

Fast Water Transport in UTSA-280 via a Knock-Off Mechanism.

Water and other small molecules frequently coordinate within metal-organic frameworks (MOFs). These coordinated molecules may actively engage in mass transfer, moving together with the transport molecules, but this phenomenon has yet to be examined. In this study, we explore a unique water transfer mechanism in UTSA-280, where an incoming water molecule can displace a coordinated molecule for mass transfer.

Pachyvitelliform maculopathy: an optical coherence tomography analysis of a novel entity.

Purpose: To describe the optical coherence tomography features of pachyvitelliform maculopathy (PVM), an acquired vitelliform lesion (AVL) associated with pachychoroid disease.

Methods: This study was a retrospective, multicentre, observational analysis.Medical records and multimodal imaging were reviewed in all patients with pachychoroid disease and AVL.

Mixed-linker strategy for suppressing structural flexibility of metal-organic framework membranes for gas separation.

Structural flexibility is a critical issue that limits the application of metal-organic framework (MOF) membranes for gas separation. Herein we propose a mixed-linker approach to suppress the structural flexibility of the CAU-10-based (CAU = Christian-Albrechts-University) membranes. Specifically, pure CAU-10-PDC membranes display high separation performance but at the same time are highly unstable for the separation of CO/CH.

Degradation mechanism of aflatoxin B1 and aflatoxin G1 by salt tolerant Bacillus albus YUN5 isolated from 'doenjang', a traditional Korean food.

Aflatoxins are the mycotoxins that contaminate food and feed and pose health hazards to humans and animals. Here, Bacillus albusYUN5 was isolated from doenjang (Korean fermented soybean paste) and examined for aflatoxin B1 (AFB1) and aflatoxin G1 (AFG1) degradation capabilities. The highest degradation of AFB1 (76.

Challenges in Developing MOF-Based Membranes for Gas Separation.

Metal-organic frameworks (MOFs) are promising candidates for membrane gas separation. MOF-based membranes include pure MOF membranes and MOF-based mixed matrix membranes (MMMs). This Perspective discusses the challenges for the next stage of the development of MOF-based membranes based on research conducted in the past decade.

Correlation between the microbiome and pack burst spoilage of Allium sativum supplemented fermented hot pepper paste.

Gochujang (fermented hot pepper paste) products are well known for their distinct, spicy flavor. However, frequent pack burst spoilage of gochujang products occurs during transportation and storage because of microbial aerogenesis, resulting in considerable economic losses. The present study aimed to prevent pack burst spoilage of gochujang products by supplementing them with garlic ethanol extract.

Extract Regulates Neuroinflammation In Vitro and In Vivo.

Previously, we reported that () is a brown algae species that exerts anti-inflammatory activity toward murine macrophages. However, the anti-neuroinflammatory effects and the mechanism of on microglia cells are still unknown. We investigated the anti-neuroinflammatory effects of extract on microglia in vitro and in vivo.

Statistical discrimination using different machine learning models reveals dissimilar key compounds of soybean leaves in targeted polyphenol-metric metabolomics in terms of traits and cultivation.

Soybean (SB) leaves (SLs) contain diverse flavonoids with health-promoting properties. To investigate the chemical constituents of SB and their correlations across phenotypes, growing periods, and environmental factors, a validated separation method for mass detection was used with targeted metabolomics. Thirty-six polyphenols (1 coumestrol, 5 flavones, 18 flavonols, and 12 isoflavones) were identified in SLs, 31 of which were quantified.

A novel glucagon analog with an extended half-life, HM15136, normalizes glucose levels in rodent models of congenital hyperinsulinism.

Congenital hyperinsulinism (CHI) is a rare genetic condition characterized by uncontrolled insulin secretion, resulting in hypoglycemia. Although glucagon has lately been regarded as a therapeutic option for CHI, its use is severely hampered by its poor solubility and stability at physiological pH, as well as its short duration of action. To address these constraints, we developed HM15136, a novel long-acting glucagon analog composed of a glucagon analog conjugated to the Fc fragment of human immunoglobulin G4 via a polyethylene glycol linker.