Agar/gelatin hydro-film containing EGF and Aloe vera for effective wound healing.

In the current study, we produced a hydro-film dressing for the treatment of chronic wounds. The hydro-film structure was composed of gelatin cross-linked with citric acid, agar and Aloe vera extract (AV); additionally epidermal growth factor (EGF) was loaded to promote wound healing. Due to the excellent hydrogel-forming ability of gelatin, the obtained hydro-film was able to swell 884 ± 36% of its dry weight, which could help controlling wound moisture.

Valorization of agar production residue as a filler in soy protein hydrogels for 3D printing.

Cellulose-containing residue from agar production was incorporated as a filler into soy protein-based hydrogels and revalorized without further purification. Rheological assessment of these hydrogels was carried out in order to confirm their shear-thinning behavior and their suitability for 3D printing. It was observed that all hydrogels behaved as weak gels, which are suitable for 3D printing and have good printability and shape fidelity.

Combination of 3D printing and electrospinning to develop chitin/gelatin/PVA scaffolds.

In this study, novel scaffolds based on natural polymers were developed by combining 3D printing (3DP) and electrospinning (ES) techniques. ES ink was prepared with gelatin and poly(vinyl alcohol) (PVA), while 3DP ink was prepared with gelatin and chitin. Different biopolymers were used to confer unique properties to each ink and obtain a multilayered scaffold suitable for tissue regeneration.

Green hemostatic sponge-like scaffold composed of soy protein and chitin for the treatment of epistaxis.

Epistaxis is one of the most common otorhinolaryngology emergencies worldwide. Although there are currently several treatments available, they present several disadvantages. This, in addition to the increasing social need of being environmentally respectful, led us to investigate whether a sponge-like scaffold (SP-CH) produced from natural by-products of the food industry - soy protein and β-chitin - can be employed as a nasal pack for the treatment of epistaxis.

Compression Molded Soy Protein Films with Exopolysaccharides Produced by Cider Lactic Acid Bacteria.

Two exopolysaccharide (EPS)-producing lactic acid bacteria (LAB) strains, CUPV281 and CUPV271, were isolated from Spanish apple must. Each of the strains produced a dextran, with different branching degrees, to be incorporated into soy protein isolate (SPI) film-forming formulations. Films were prepared by compression molding, a more rapid processing method than solution casting and, thus, with a greater potential for scaling-up production.

The Effect of Cross-Linking with Citric Acid on the Properties of Agar/Fish Gelatin Films.

The aim of this work was to assess the effect of fish gelatin-citric acid nucleophilic substitution and agar-citric acid esterification reactions on the properties of agar/fish gelatin films. Since temperature is an important cross-linking parameter, films were treated at 90 °C and 105 °C and film properties were compared to those of non-cured films. It was observed that temperature favored the aforementioned reactions, which induced physical and morphological changes.

Valorization of marine-derived biowaste to develop chitin/fish gelatin products as bioactive carriers and moisture scavengers.

Marine-derived biowaste was valorized to develop chitin/fish gelatin porous materials with the aim of being used as moisture scavengers and bioactive carriers. Chitin was extracted from squid pens, abundant and available biowastes from fishery industry, through a sustainable process and the environmental assessment was carried out. Besides the valorization of biowaste, it is worth noting that the use of this specific biowaste allows the avoidance of discoloration and demineralization processes to extract chitin and, thus, a lower consumption of resources, both chemicals and energy, in comparison to the conventional chitin extraction from crustacean shells.

Electrospinning of Fish Gelatin Solution Containing Citric Acid: An Environmentally Friendly Approach to Prepare Crosslinked Gelatin Fibers.

The majority of the crosslinking approaches employed to confer water resistance properties to electrospun gelatin mats are based on the use of potential cytotoxic agents, turning out to be not suitable for biomedical applications. Environmentally friendly chemical strategies based on the use of non-toxic agents are, therefore, strongly demanded. In the present work, the possibility to produce crosslinked electrospun fish gelatin mats by electrospinning an aqueous solution, containing citric acid as a crosslinking agent, is reported.

Development of Bioinspired Gelatin and Gelatin/Chitosan Bilayer Hydrofilms for Wound Healing.

In the current study, we developed a novel gelatin-based bilayer wound dressing. We used different crosslinking agents to confer unique properties to each layer, obtaining a bioinspired multifunctional hydrofilm suitable for wound healing. First, we produced a resistant and non-degradable upper layer by lactose-mediated crosslinking of gelatin, which provided mechanical support and protection to overall design.

Extraction and incorporation of bioactives into protein formulations for food and biomedical applications.

Bioactive compounds from natural resources have been pointed out as promising substances with great benefits for human health. Furthermore, new techniques for their extraction have been reviewed in order to consider a global approach taking economic and environmental issues into account. Additionally, the incorporation of these biologically active compounds into protein-based products manufactured via different processing methods, such as solution casting, compression moulding, electrospinning, freeze-drying, and 3D printing, has been assessed, since the design and manufacturing method employed have a direct influence into the final properties of the material.

Valorisation of blueberry waste and use of compression to manufacture sustainable starch films with enhanced properties.

Blueberry waste from juice processing was valorised to develop starch films by compression moulding. The compression process resulted in hydrophobic films with water contact angles even higher than 100° for the films prepared with the highest blueberry waste content. Additionally, the film solubility was reduced by the incorporation of blueberry waste, regardless of the solution pH.