Publications by authors named "Jonathan R Hudson"
Most organs have tissue-resident immune cells. Human organoids lack these immune cells, which limits their utility in modeling many normal and disease processes. Here, we describe that pluripotent stem cell-derived human colonic organoids (HCOs) co-develop a diverse population of immune cells, including hemogenic endothelium (HE)-like cells and erythromyeloid progenitors that undergo stereotypical steps in differentiation, resulting in the generation of functional macrophages.
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- Functional assays are crucial for determining the impact of germline variants in DNA mismatch repair genes, particularly in MLH1, which is linked to Lynch syndrome.
- The study created a new human cell-based method to test variants of uncertain significance (VUS) in the MLH1 gene by using CRISPR to integrate these variants into embryonic stem cells and assess their effects on RNA, protein, and DNA stability.
- By analyzing the functional results through statistical methods, researchers aim to provide insights that can help expert panels better classify VUS and improve diagnosis and treatment for Lynch syndrome patients.
Gastric and small intestinal organoids differentiated from human pluripotent stem cells (hPSCs) have revolutionized the study of gastrointestinal development and disease. Distal gut tissues such as cecum and colon, however, have proved considerably more challenging to derive in vitro. Here we report the differentiation of human colonic organoids (HCOs) from hPSCs.
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