Publications by authors named "Jonathan Paules"
The tardigrade Hypsibius exemplaris is an emerging model organism renowned for its ability to survive environmental extremes. To explore the molecular mechanisms and genetic basis of such extremotolerance, many studies rely on RNA-sequencing (RNA-seq), which can be performed on populations ranging from large cohorts to individual animals. Reverse transcription polymerase chain reaction (RT-PCR) and RNA interference (RNAi) are subsequently used to confirm RNA-seq findings and assess the genetic requirements for candidate genes, respectively.
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- Tardigrades are remarkable organisms known for surviving extreme environments, making them a key focus for studying genetic mechanisms behind their resilience.
- RNA-sequencing (RNA-seq) and techniques like RT-PCR and RNA interference are used to analyze their genetic responses, but require efficient RNA extraction methods.
- This study introduces a new single-tardigrade RNA extraction method (STST) that simplifies the process, reduces costs, and allows for effective analysis of gene expression related to heat shock proteins using qRT-PCR.