Surgery and postoperative definitive radiotherapy for management of canine soft tissue sarcoma: a multi-institutional retrospective study of 272 dogs (2010-2020).

Objective: To report local progression and survival in dogs following surgery and postoperative definitive radiotherapy (dRT) for management of soft tissue sarcoma (STS) and to evaluate risk factors for local progression and survival.

Methods: Records were retrospectively reviewed at 9 referral hospitals for dogs managed with postoperative dRT between January 1, 2010, and January 1, 2020, following surgery for STS. Data related to presentation, surgery, dRT, systemic therapy, and outcome were abstracted.

Craniectomies for Dogs With Skull Multilobular Osteochondrosarcoma Using the Misonix Bone Scalpel: Cadaveric Evaluation and Retrospective Case Series.

To evaluate the Misonix bone scalpel (MBS) for craniotomies in dogs and describe clinical findings and surgical experience in 3 dogs with large multilobular osteochondrosarcoma (MLO) of the skull. Cadaver evaluation and retrospective case series. One canine cadaver; 3 client-owned dogs.

[Not Available].

A synthetic peptide was found to block cell-to-cell signalling, or quorum sensing, in bacteria and be highly bioavailable in mouse tissue. The controlled release of this agent from degradable polymeric microparticles strongly inhibited skin infection in a wound model at levels that far surpassed the potency of the peptide when delivered conventionally.

The development of ventricular fibrillation as a complication of pericardiectomy in 16 dogs.

Objective: To describe the clinical characteristics, perioperative protocols, and outcomes in dogs diagnosed with ventricular fibrillation (VF) while undergoing pericardiectomy.

Study Design: Retrospective, multi-institutional study.

Animals: Sixteen client-owned dogs.

Bifunctional Peptide that Anneals to Damaged Collagen and Clusters TGF-β Receptors Enhances Wound Healing.

Transforming growth factor-β (TGF-β) plays important roles in wound healing. The activity of TGF-β is initiated upon the binding of the growth factor to the extracellular domains of its receptors. We sought to facilitate the activation by clustering these extracellular domains.

Retroperitoneal fibrosis as a postoperative complication following renal transplantation in cats.

Objectives: The aim of this report was to describe the clinical signs, diagnostic imaging findings, surgical management, histopathological findings, outcome and possible risk factors for cats that developed retroperitoneal fibrosis (RPF) following renal transplantation.

Methods: Medical records of cats that underwent renal transplantation and developed clinically significant RPF between 1995 and 2019 were reviewed.

Results: Eighty-one cats underwent 83 renal transplantations.

Dorsal offset rhinoplasty for treatment of stenotic nares in 34 brachycephalic dogs.

Objective: To describe the technique, outcome, and owner satisfaction associated with dorsal offset rhinoplasty (DOR) to treat stenotic nares in brachycephalic dogs.

Study Design: Retrospective case series.

Animals: Thirty-four client-owned dogs.

Catheter occlusion of the dorsal sagittal sinus-confluens sinuum to enable resection of lateral occipital multilobular osteochondrosarcoma in two dogs.

Case Description: A 6-year-old neutered male mixed-breed dog (dog 1) and 5-year-old neutered male Boston Terrier (dog 2) were evaluated because of lateralized multilobular osteochondrosarcoma (MLO) of the occiput.

Clinical Findings: Diagnostic imaging revealed proliferative bony occipital masses in both dogs and a nodule in the right caudal lung lobe of dog 1. For both dogs, MRI revealed intact flow through the dorsal sagittal sinus (DSS) into 1 transverse sinus.

Novel murine model for delayed wound healing using a biological wound dressing with Pseudomonas aeruginosa biofilms.

Bacterial biofilms impair healing in 60% of chronic skin wounds. Various animal models (mice, rats, rabbits, and pigs) have been developed to replicate biofilm infected wounds in vivo. We developed a sustained wound infection model by applying preformed Pseudomonas aeruginosa biofilms on a wound dressing to full-thickness murine skin wounds.

Pilot Study of a "Large-Eye," Surgically Induced Dry Eye Rabbit Model by Selective Removal of the Harderian, Lacrimal, and Meibomian Glands.

Background/aims: Establish a reliable rabbit dry eye (DE) model.

Methods: An interventional cohort study surgically removing glands contributing to the tear film. Eight rabbits were studied after removal of left lacrimal, Harderian, or both glands.

A Carrel patch technique for renal transplantation in cats.

Objective: To evaluate the feasibility of a Carrel patch method in feline renal transplantation.

Study Design: Descriptive case series.

Animals: Nine healthy donor cats and 9 client recipient cats with chronic renal failure.

Effect of cold storage on immediate graft function in an experimental model of renal transplantation in cats.

OBJECTIVE To assess the effect of cold storage (CS) on immediate posttransplantation function of renal autografts in cats. ANIMALS 15 healthy 1-year-old cats. PROCEDURES Cats were assigned to 2 groups and underwent autotransplantation of the left kidney followed by nephrectomy of the right kidney.

Interfacial Stacks of Polymeric Nanofilms on Soft Biological Surfaces that Release Multiple Agents.

We report a general and facile method that permits the transfer (stacking) of multiple independently fabricated and nanoscopically thin polymeric films, each containing a distinct bioactive agent, onto soft biomedically relevant surfaces (e.g., collagen-based wound dressings).

Ureteral Papilla Implantation as a Technique for Neoureterocystostomy in Cats Undergoing Renal Transplantation: 30 Cases.

Objective: To describe the clinical outcome of donor and recipient cats undergoing ureteral papilla harvest and implantation as a technique for neoureterocystostomy in clinical kidney transplant.

Study Design: Retrospective case series.

Animals: Donor (n=31) and recipient (n=30) cats that underwent kidney harvest and transplantation using ureteral papilla implantation technique for neoureterocystostomy.

Gallium-Loaded Dissolvable Microfilm Constructs that Provide Sustained Release of Ga(3+) for Management of Biofilms.

The persistence of bacterial biofilms in chronic wounds delays wound healing. Although Ga(3+) can inhibit or kill biofilms, precipitation as Ga(OH)3 has prevented its use as a topical wound treatment. The design of a microfilm construct comprising a polyelectrolyte film that releases noncytotoxic concentrations of Ga(3+) over 20 d and a dissolvable micrometer-thick film of polyvinylalcohol that enables facile transfer onto biomedically important surfaces is reported.

Vascular hamartoma in the right ventricle of a dog: Diagnosis and treatment.

A 6-month old Labrador retriever was presented with an acute history of collapse during exercise. A grade III/VI left basilar systolic murmur and thoracic radiographs showing severe right heart enlargement with an enlarged main pulmonary artery were most consistent with a clinical diagnosis of pulmonic stenosis. Echocardiography revealed an intracardiac mass partially obstructing the right ventricular outflow tract.

Inhibition of Pseudomonas aeruginosa biofilm formation on wound dressings.

Chronic nonhealing skin wounds often contain bacterial biofilms that prevent normal wound healing and closure and present challenges to the use of conventional wound dressings. We investigated inhibition of Pseudomonas aeruginosa biofilm formation, a common pathogen of chronic skin wounds, on a commercially available biological wound dressing. Building on prior reports, we examined whether the amino acid tryptophan would inhibit P.

Outcome of Donor Cats After Unilateral Nephrectomy as Part of a Clinical Kidney Transplant Program.

Objective: To compare 1) complications between 2 ureteral harvest techniques (ureteral papilla harvest [UPH] and ureteral transection [UT]); 2) to investigate the prevalence of kidney failure in a population of kidney donors; and 3) to evaluate owner satisfaction with commercially sourced cats adopted after kidney donation.

Study Design: Retrospective case series.

Animals: Cats (n = 72) that had unilateral nephrectomy for kidney donation.

Importance of defining experimental conditions in a mouse excisional wound model.

The murine dorsum dermal excisional wound model has been widely utilized with or without splint application. However, variations in experimental methods create challenges for direct comparison of results provided in the literature and for design of new wound healing studies. Here, we investigated the effects of wound location and size, number of wounds, type of adhesive used for splint fixation on wound healing using splinted or unsplinted dorsum excisional full thickness wound models.

PDGF-BB does not accelerate healing in diabetic mice with splinted skin wounds.

Topical application of platelet-derived growth factor-BB (PDGF-BB) is considered to accelerate tissue repair of impaired chronic wounds. However, the vast literature is plagued with conflicting reports of its efficacy in animal models and this is often influenced by a wide array of experimental variables making it difficult to compare the results across the studies. To mitigate the confounding variables that influence the efficacy of topically applied PDGF-BB, we used a controlled full thickness splinted excisional wound model in db/db mice (type 2 diabetic mouse model) for our investigations.

Diagnosis and surgical treatment of a malignant trichoepithelioma of the ear canal in a pet rabbit (Oryctolagus cuniculus).

Case Description: A 10-year-old spayed female Holland Lop-mix pet rabbit (Oryctolagus cuniculus) was evaluated because of purulent-hemorrhagic discharge from the right ear canal and a suspected mass within that ear canal.

Clinical Findings: Results of contrast-enhanced CT, video otoscopy, and histologic examination of endoscopic tissue biopsy samples indicated severe otitis media and externa and a benign trichoepithelioma of the right ear canal.

Treatment And Outcome: Total ear canal ablation and lateral bulla osteotomy were performed.

Full-thickness splinted skin wound healing models in db/db and heterozygous mice: implications for wound healing impairment.

The excisional dorsal full-thickness skin wound model with or without splinting is widely utilized in wound healing studies using diabetic or normal mice. However, the effects of splinting on dermal wound healing have not been fully characterized, and there are limited data on the direct comparison of wound parameters in the splinted model between diabetic and normal mice. We compared full-thickness excisional dermal wound healing in db/db and heterozygous mice by investigating the effects of splinting, semi-occlusive dressing, and poly(ethylene glycol) treatment.

Anchoring a cytoactive factor in a wound bed promotes healing.

Wound healing is a complex process that requires the intervention of cytoactive factors. The one-time application of soluble factors to a wound bed does not maintain a steady, sufficient concentration. Here we investigated the benefits of anchoring a factor in a wound bed via a tether to endogenous collagen.

Raman spectroscopy enables noninvasive biochemical characterization and identification of the stage of healing of a wound.

Accurate and rapid assessment of the healing status of a wound in a simple and noninvasive manner would enable clinicians to diagnose wounds in real time and promptly adjust treatments to hasten the resolution of nonhealing wounds. Histologic and biochemical characterization of biopsied wound tissue, which is currently the only reliable method for wound assessment, is invasive, complex to interpret, and slow. Here we demonstrate the use of Raman microspectroscopy coupled with multivariate spectral analysis as a simple, noninvasive method to biochemically characterize healing wounds in mice and to accurately identify different phases of healing of wounds at different time-points.