Publications by authors named "Jonathan H Brumbach"
The clinical success of non-viral gene delivery reagents is hampered by their inefficient cellular transgene delivery, which is largely influenced by carrier properties that are currently undefined and misunderstood. In an attempt to further define and understand the requirements for a safe and efficient non-viral gene delivery reagent, research labs often engineer and evaluate many putative products with subtle physiochemical differences in order to delineate requirements for improved in vitro and in vivo success. The synthesis of many putative reagents is often time-intensive, laborious and costly.
View Article and Find Full Text PDFThe pathogenesis of type-1 diabetes is complicated, and a clear, single mechanism has yet to be identified. Reports have indicated that the activating receptor NKG2D plays an important role in the development of disease. Exploiting a natural phenomenon observed in tumors, plasmid DNA encoding for a soluble ligand to NKG2D (sRAE-1γ) was isolated and engineered into a plasmid expression system.
View Article and Find Full Text PDFMechanism of sodium uptake in PNA negative MR cells from rainbow trout, Oncorhynchus mykiss as revealed by silver and copper inhibition.
Comp Biochem Physiol A Mol Integr Physiol
July 2011
Article Synopsis
- The study measured sodium uptake in different cell types from rainbow trout gills, finding that Na(+) transport in pavement cells was unaffected by acidification, while Na(+) uptake in peanut lectin agglutinin-negative mitochondrion-rich cells increased significantly under acidic conditions.
- Acid-stimulated Na(+) uptake in these cells was completely blocked by specific inhibitors phenamil and bafilomycin, as well as metals like silver and copper, which did not affect the basal Na(+) transport.
- The results suggest that peanut lectin agglutinin-negative mitochondrion-rich cells are crucial for Na(+) uptake in freshwater trout gills, highlighting the role of protons in Na(+) movement regulation.
Branched disulfide-containing poly(amido ethyleneimines) (SS-PAEIs) are biodegradable polymeric gene carrier analogues of the well-studied, nondegradable, and often toxic branched polyethylenimines (bPEIs), but with distinct advantages for cellular transgene delivery. Clinical success of polycationic gene carriers is hampered by obscure design and formulation requirements. This present work reports synthetic and formulation properties for a graft copolymer of poly(ethylene glycol) (PEG) and a branched SS-PAEI, poly(triethylentetramine/cystaminebisacrylamide) (p(TETA/CBA)).
View Article and Find Full Text PDFA bioreducible poly(amido amine) (SS-PAA) gene carrier, known as poly (amido-butanol) (pABOL), was used to transfect a variety of cancer and non-cancer cell lines. To obtain cancer-specific transgene expression for therapeutic efficiency in cancer treatment, we constructed survivin-inducible plasmid DNA expressing the soluble VEGF receptor, sFlt-1, downstream of the survivin promoter (pSUR-sFlt-1). Cancer-specific expression of sFlt-1 was observed in the mouse renal carcinoma (RENCA) cell line.
View Article and Find Full Text PDF