Gene Regulatory Alleles and Reporter Bashing Studies.

Gene regulation has been studied in for over 30 years. In this analysis of 102 publications, we find that most transcriptional cis-regulatory elements are located within 5,000 bp of the transcription start site. Over 75% of studies conclude that transcriptional elements and 5'UTRs activate-, while 3'UTRs repress gene expression.

Parallel genetics of regulatory sequences using scalable genome editing in vivo.

How regulatory sequences control gene expression is fundamental for explaining phenotypes in health and disease. Regulatory elements must ultimately be understood within their genomic environment and development- or tissue-specific contexts. Because this is technically challenging, few regulatory elements have been characterized in vivo.

Estimation of cell- and tissue volumes.

Although is one of the best-studied model organisms, an estimate of its cell sizes and tissues is missing. Here we used the Virtual Worm that is based on electron microscopy images to calculate a zeroth-order approximation of cell and tissue sizes of . We conclude that the intestine is the largest tissue, followed by the hypodermis, gonads, body wall muscles, pharynx, and neurons.