helicase fails to complement loss of Prp43p in .

Helicase proteins have important roles in many aspects of RNA metabolism in the cell. The function of these highly conserved proteins is commonly preserved between organisms, yet in a few cases these homologues are found to have slightly different biochemical functions. Prp43 is a protein with varied roles in yeast, but here we show that the homologue of this protein is unable to rescue the loss of Prp43p.

Genetics of yeast impacting wine quality.

The availability of the sequence of the Saccharomyces genome in combination with the development of chemical analytical technologies with dynamic ranges sensitive enough to detect volatile aromatic compounds has generated a renewed interest in defining the role of yeast in the generation of wine aroma and flavor. Genetic differences among wine strains are well documented and aroma profiles also appear to vary, implying that specific allelic alterations may exist and impact the production of compounds associated with flavor. Partial or complete sequencing data on several wine strains are available and reveal underlying genetic differences across strains in key genes implicated in flavor formation.

Functional genomics of wine yeast Saccharomyces cerevisiae.

The application of genomic technologies to the analysis of wine strains of Saccharomyces cerevisiae has greatly enhanced our understanding of both native and laboratory strains of this important model eukaryote. Not only are differences in transcript, protein, and metabolite profiles being uncovered, but the heritable basis of these differences is also being elucidated. Although some challenges remain in the application of functional genomic technologies to commercial and native strains of S.

Aquaporins in Saccharomyces cerevisiae wine yeast.

AQY1 and AQY2 were sequenced from five commercial and five native wine yeasts. Of these, two AQY1 alleles from UCD 522 and UCD 932 were identified that encoded three or four amino-acid changes, respectively, compared with the Sigma1278b sequence. Oocytes expressing these AQY1 alleles individually exhibited increased water permeability vs.