New strategy for RDC-assisted diastereotopic proton assignment using a combination of J-scaled BIRD HSQC and J-scaled BIRD HMQC/HSQC.

A new strategy to assign diastereotopic protons was developed on the basis of residual dipolar couplings (RDCs) collected in compressed poly(methyl methacrylate) (PMMA) gels. A combination of 2D J-scaled BIRD HSQC and J-scaled BIRD HMQC/HSQC NMR experiments was used to collect the RDC data. In the proposed strategy, the first experiment is used to measure (1)D(CH) for methine groups, the sum of (1)D(CHa) + (1)D(CHb) for methylene groups and the average (1)D(CH3) value for methyl groups.

Expression of the Helicobacter pylori adhesin SabA is controlled via phase variation and the ArsRS signal transduction system.

Adaptation to the acidic microenvironment, and adherence to mucosal epithelium, are essential for persistent colonization of the human stomach by Helicobacter pylori. The expression of SabA, an adhesin implicated in the ability of H. pylori to adhere to the host gastric epithelium, can be modulated by phase variation via slipped-strand mispairing in repetitive nucleotide tracts located in both the promoter region and the coding region.