This work focuses on two new freshwater crab species from Tanzania, East Africa: Arcopotamonautes parekeeae n. sp. and A.
View Article and Find Full Text PDFThe aim of this study was to investigate the effect of watering before slaughter and different evisceration methods on the quality characteristics of fattened goose liver. A total of randomly selected 60 male Greylag Landaise geese (Anser anser) were used to execute the experiment which had been force-fed during their growing period. In the slaughterhouse, randomized block design was followed and the birds were randomly divided into four experimental groups and half of the them (30 birds) were given 200 ml tap water.
View Article and Find Full Text PDFIn this study combined effect of ultrasound-induced acoustic cavitation and microbubbles during meat brining on pork loin (Longissimus dorsi) was evaluated. Cylindrical shape (diameter 15 mm, height 80 mm) pork loin samples were cut and immersed in 200 g L NaCl brine and treated with the following brining methods for 180 min: static brining (SB), ultrasound assisted brining (US) and ultrasound combined with microbubbles in brine (USMB). Ultrasound was generated with 20 kHz frequency, 5,09 W/cm maximum intensity and 100 W maximum power.
View Article and Find Full Text PDFUnlabelled: Összefoglaló. Bevezetés: Az emberi sziklacsont a halántékcsont része, egy bonyolult és változatos anatómiai felépítésű struktúra. A sziklacsonton végzett beavatkozások előtt, a műtéti szövődmények megelőzése érdekében, nélkülözhetetlen a biztos anatómiai tudás és kézügyesség megszerzése, valamint az egyes műtéti lépések és mozdulatok begyakorlása.
View Article and Find Full Text PDFAim: The first survey of the German Society of Nuclear Medicine (DGN) to assess the resources of our speciality named "Inventory Resources of Nuclear Medicine" aims to provide a reliable data basis for existing and future activities of the DGN. The involvement and participation of as many colleagues as possible will play an important role for the success of the development of our profession.
Methods: 214 leading nuclear medicine professionals were identified as the target group.
Aim: To assess available e-learning concepts and programmes for nuclear medicine at university hospitals in Germany.
Methods: All (34) departments of nuclear medicine at German university hospitals were asked to participate in an anonymized online survey. Questions were categorized into four topics: 1.
Diagn Microbiol Infect Dis
November 2008
The ARCHITECT Toxo IgG and IgG Avidity assays have been developed as a fully automated panel for immune status determination and acute infection exclusion. Resolved relative specificity and sensitivity of the ARCHITECT Toxo IgG assay were 99.6% (1359/1365) and 99.
View Article and Find Full Text PDFBackground: Before the introduction of human immunodeficiency virus (HIV) combination assays, serologic diagnosis of HIV infection was performed with assays that detected either antibodies or p24 antigen. Owing to the capability to detect the early appearance of p24 antigen, combination assays that are designed for simultaneous detection of antibodies and antigen can significantly reduce the diagnostic window.
Study Design And Methods: Specificity and sensitivity of a commercially available HIV antigen-antibody combination assay (Abbott PRISM; assay is not licensed by the FDA for use in the United States) were evaluated in a multicenter study by testing volunteer blood donors, hospitalized patients, seroconversion panels, and p24 antigen and HIV antibody subtype panels.
Background And Objectives: Compliance with current regulations regarding the prevention of hepatitis C virus (HCV) transmission in the blood transfusion setting requires the use of sensitive assays for HCV antibody (anti-HCV) detection, which should, ideally, identify any donor having had prior contact with the virus. Therefore, low-level anti-HCV positive blood units should be detected by the screening assays, even those reflecting a past and resolved infection. To assess the sensitivity of two versions of an automated chemiluminescent microparticle immunoassay (CMIA) for anti-HCV screening (ARCHITECT Anti-HCV), 113 single serum samples containing low levels of anti-HCV, assessed by two immunoblot tests, were selected from 3686 samples received for confirmation of HCV infection by a reference laboratory over a 2-year period.
View Article and Find Full Text PDFIntroduction: The ARCHITECT Anti-HCV assay is a fully automated high throughput chemiluminescent microparticle immunoassay (CMIA) for the detection of antibodies to structural and nonstructural proteins of the hepatitis C virus (HCV). To further enhance the performance of this test, the assay was modified to improve the specificity for blood donor specimens.
Methods: The specificity of the enhanced ARCHITECT Anti-HCV assay was evaluated by screening blood donor samples randomly collected from various German blood banks, as well as hospitalized patient samples derived from Germany and the US.
Pharmaceutical companies have a continuous need to resolve new racemates. Analysis may be required in aqueous and nonaqueous media, or in the presence of several different sets of potentially interfering compounds. There is often a preparative requirement.
View Article and Find Full Text PDFJ Chromatogr B Analyt Technol Biomed Life Sci
March 2003
A DNA fragment coding for the human CD4 cytoplasmic domain (residues 394-433) was cloned into the pET15b expression vector. The resulting plasmid was used for synthesis of the polyhistidine-tagged 5.10(3) M(r) CD4 peptide in Escherichia coli BL21(DE3)Star.
View Article and Find Full Text PDFJ Biomed Sci
September 2001
The transactivator protein (Tat) of the human immunodeficiency virus (HIV) is a key regulatory protein in the viral replication cycle. Together with cellular cyclin T1 and an RNA element (transactivation response; TAR) located at the 5' end of all viral transcripts, it forms a ternary complex that ultimately enhances the expression of all viral genes. In this ternary complex, cyclin T1 interacts directly with Tat and TAR.
View Article and Find Full Text PDFAn evaluation of the use of the Jasco CD-995 in conjunction with achiral high-performance liquid chromatography (HPLC) for the determination of the enantiomeric purity of various SB compounds is described. The Jasco CD-995 is the first commercially-available detector specifically designed for the on-line monitoring of circular dichroism (CD) at a fixed wavelength. Predecessors such as the OR-990 from Jasco and other manufacturers use optical rotation as the detection principle.
View Article and Find Full Text PDFThe atrial natriuretic peptide hormonal system is altered to a variable degree in patients with cirrhosis. Portal pressure and portal-systemic shunting are also varied in cirrhosis. We used a portal vein-ligated rat model with predictable portal hypertension to study the effects of portal hypertension alone on the atrial natriuretic peptide hormonal system.
View Article and Find Full Text PDFAtrial natriuretic factor (ANF) is a cardiac hormone with potent natriuretic, diuretic, and vasorelaxant properties. Although abnormalities in ANF release, plasma level, and renal receptors have been described in humans and/or animals with cirrhosis and ascites, little is known about the ANF hormonal system in cirrhosis without ascites. The aim of this study was to examine the ANF hormonal system in an animal model of cirrhosis to determine whether compensated cirrhosis is associated with changes in the ANF hormonal system.
View Article and Find Full Text PDFA year ago, the nurses of the 18th Airborne COSCOM, 44th Med Brigade, 5th Mobile Army Surgical Hospital Unit, and the 46th Combat Support Hospital with the 24th Infantry Division arrived in the Saudi Arabian desert. Their experiences left some of them challenging accepted stateside nursing procedures.
View Article and Find Full Text PDFTo test whether omeprazole would increase the susceptibility of the duodenum to damage, 200 to 250-g male Sprague-Dawley rats were given 10 mg/kg of omeprazole (Losec) by gavage every morning for 29 days. Control rats were given gavage buffer alone. After fasting overnight, half the rats received 10 mg/kg indomethacin intraperitoneally; then all rats were given 2 ml of 50% ethanol by gavage.
View Article and Find Full Text PDFAliment Pharmacol Ther
February 1991
The purpose of this study was to determine whether portal hypertension potentiates intestinal ulceration induced by indomethacin. Portal hypertension was produced in male Sprague-Dawley rats by two-staged ligation of the portal vein. Sham-operated rats were used as controls.
View Article and Find Full Text PDFThis study's purpose was to determine whether portal hypertension adversely affects small intestinal mucosal injury. Portal hypertension was produced in male Sprague-Dawley rats by two-stage ligation of the portal vein. Sham-operated rats were used as controls.
View Article and Find Full Text PDFRational treatment of portal hypertensive complications requires a knowledge of the cause of portal hypertension and an assessment of the severity of liver disease. In the United States, chronic liver disease, usually due to alcohol, is the most common underlying cause. The history, physical examination, and laboratory analysis are usually sufficient to confirm the presence of underlying liver disease.
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