is the most common human fungal pathogen, able to reside in a broad range of niches within the human body. Even though systemic infection is associated with high mortality, the fungus has historically received relatively little attention, resulting in a lack of optimized molecular and fluorescent tools. Over the last decade, some extra focus has been put on the optimization of fluorescent proteins (FPs) of C.
View Article and Find Full Text PDFFluorescent proteins (FPs) have always been a crucial part of molecular research in life sciences, including the research into the human fungal pathogen but have obvious shortcomings such as their relatively large size and long maturation time. However, the next generation of FPs overcome these issues and rely on the binding of a fluorogen for the protein to become fluorescently active. This generation of FPs includes the improved version of Fluorescence activating and Absorption Shifting Tag (iFAST).
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