Characterization of the virus of sialodacryoadenitis of rats: a member of the coronavirus group.

The virus that causes sialodacryoadenitis in rats has been isolated in mice and in primary cultures of rat-kidney cells and has been characterized as a heat-labile RNA virus that is sensitive to lipid solvents and is relatively stable at H 3.0. This virus is antigenically related to the virus of hepatitis in mice and to coronavirus of rats.

Experimental Brucella canis infection in the monkey (Macaca arctoides).

Four stumptail Macaque monkeys (Macaca arctoides) were each inoculated with approximately 10(10) organisms from a culture of Brucella canis. Two animals were inoculated via the oral and conjunctival route and the other two monkeys were inoculated intravenously with the organisms. A fifth animal served as a control.

Rapid detection of bacteremia in mice by gas chromatography.

Gas chromatography of the serum of mice inoculated 6 hr earlier with Staphylococcus aureus, Escherichia coli K-12, and Clostridium chauvoei revealed the formation of products associated with the infections. Longer periods were required before compounds resulting from infecting the animals with Salmonella typhimurium and other Clostridium species were detected by gas chromatographic means. The technique is sensitive to the presence of metabolites elaborated in vivo as a result of the presence of 100 to 35,000 cells.

Biochemical changes in serum of pure and mixed Streptococcus faecalis and Bacillus subtilis infections in rats.

Rats inoculated with Streptococcus faecalis developed endocarditis and demonstrated a 6- to 30-fold increase in aldolase, isocitric dehydrogenase, phosphohexose isomerase, and lactic dehydrogenase. The animals infected with Bacillus subtilis did not develop overt disease nor significant increases in enzyme activities, but viable organisms were recovered at 2 weeks. Rats inoculated with mixed culture of these organisms showed a 2- to 10-fold increase of enzyme activities without evidence of pathological anatomic changes.