Ionizable Lipid Nanoparticle-Mediated TRAIL mRNA Delivery in the Tumor Microenvironment to Inhibit Colon Cancer Progression.

Introduction: Immunotherapy has revolutionized cancer treatment by harnessing the immune system to enhance antitumor responses while minimizing off-target effects. Among the promising cancer-specific therapies, tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) has attracted significant attention.

Methods: Here, we developed an ionizable lipid nanoparticle (LNP) platform to deliver TRAIL mRNA (LNP-TRAIL) directly to the tumor microenvironment (TME) to induce tumor cell death.

Nanoparticle-based DNA vaccine protects against SARS-CoV-2 variants in female preclinical models.

A safe and effective vaccine with long-term protection against SARS-CoV-2 variants of concern (VOCs) is a global health priority. Here, we develop lipid nanoparticles (LNPs) to provide safe and effective delivery of plasmid DNA (pDNA) and show protection against VOCs in female small animal models. Using a library of LNPs encapsulating unique barcoded DNA (b-DNA), we screen for b-DNA delivery after intramuscular administration.

[Eating Habits of People with Type 2 Diabetes Mellitus in Portugal: A Cross-Sectional Study].

Introduction: Nutrition is a cornerstone of diabetes mellitus prevention and management; therefore, it is essential to enable patients to adopt healthy eating habits. Previous studies have not yet documented the main errors in the eating habits of Portuguese people with type 2 diabetes mellitus. This study aims to identify the main errors in the eating habits of people living with type 2 diabetes mellitus in Portugal and to evaluate its associations with sociodemographic variables.

Small-Molecule Inhibitor of Flaviviral NS3-NS5 Interaction with Broad-Spectrum Activity and Efficacy .

Every year, dengue virus (DENV) causes one hundred million infections worldwide that can result in dengue disease and severe dengue. Two other mosquito-borne flaviviruses, i.e.

Anti-Zika Virus Activity of Plant Extracts Containing Polyphenols and Triterpenes on Vero CCL-81 and Human Neuroblastoma SH-SY5Y Cells.

Zika virus (ZIKV) infection is a global threat associated to neurological disorders in adults and microcephaly in children born to infected mothers. No vaccine or drug is available against ZIKV. We herein report the anti-ZIKV activity of 36 plant extracts containing polyphenols and/or triterpenes.

Are We Exploring the Potential Role of Specialized Techniques in Muscle Hypertrophy?

Specialized resistance training techniques (e.g., drop-set, rest-pause) are commonly used by well-trained subjects for maximizing muscle hypertrophy.

Bioaccessibility and bioavailability of calcium in sprouted brown and golden flaxseed.

Background: Germination promotes changes in the composition of seeds by providing potential nutritional and health benefits compared with unsprouted seeds. This study investigated the influence of germination on the bioaccessibility and bioavailability of calcium in brown flaxseed (BF) and golden flaxseed (GF).

Results: Germination did not influence the calcium levels of BF or GF, but the sprouted GF (SGF, 265.

Parasympathetic Nervous Activity Responses to Different Resistance Training Systems.

The assessment of parasympathetic nervous activity and psychophysiological responses infers the stress imposed by different resistance training systems. Therefore, we compare the effects of different sets configurations, with similar volume (~60 repetitions), on heart rate variability indices and internal training load. Twenty-nine resistance-trained adults completed the following conditions: traditional without and with muscle failure, inter-repetition rest, and rest-pause in the parallel squat.

Does rest interval between sets affect resistance training volume, density, and rating of perceived exertion when adopting the crescent pyramid system in young women?

Background: The rest interval between sets can affect the responses to resistance training. Thus, the purpose of this study was to compare the effects of different rest intervals (RI) on volume, density, and rating of perceived exertion (RPE) when adopting a crescent pyramid (CP) system.

Methods: Twenty young women (21.

Effect of 10% weight loss on simulated taekwondo match performance: a randomized trial.

The objective of the present study was to analyze the effect of 10% body mass reduction on simulated taekwondo match performance. Thirty-one male taekwondo athletes were randomly distributed in two groups: weight loss group (n= 15) - athletes in this group reduced 5% body mass per week during two successive weeks, totaling 10% body mass reduction; control group (n= 16) - athletes in this group kept their body mass constant during the 2-week period. Twenty-four hours before and after this period, athletes performed a simulated 3-round taekwondo match (6 min).

Comparative study by immunofluorescence of T and P antigens induced by adenovirus type 12 in permissive and nonpermissive cells.

The development of adenovirus type 12 T antigen and of the complex of antigenic early proteins designated as P antigen was studied by immunofluorescence in productively infected KB cells and abortively infected RK-13 cells. T antigen is detected in both cell types very early in infection. In KB cells it presents the well known pattern of nuclear dots and flecks but in RK-13 cells at the time of maximum abundance, 18 hours p.