Publications by authors named "Jon Oskar Wheat"
Isolation and characterization of a thermostable RNA ligase 1 from a Thermus scotoductus bacteriophage TS2126 with good single-stranded DNA ligation properties.
Thorarinn Blondal Audur Thorisdottir Unnur Unnsteinsdottir Sigridur Hjorleifsdottir Arnthor Aevarsson Jon Oskar Wheat
Nucleic Acids Res
February 2005
We have recently sequenced the genome of a novel thermophilic bacteriophage designated as TS2126 that infects the thermophilic eubacterium Thermus scotoductus. One of the annotated open reading frames (ORFs) shows homology to T4 RNA ligase 1, an enzyme of great importance in molecular biology, owing to its ability to ligate single-stranded nucleic acids. The ORF was cloned, and recombinant protein was expressed, purified and characterized.
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- A polynucleotide kinase from the RM378 bacteriophage was identified and characterized, showing both 5'-kinase and 3'-phosphohydrolase activities.
- The kinase has some sequence similarity to T4 bacteriophage's 5'-kinase but differs in the order of its domains and lacks homology in the 3'-phosphohydrolase region.
- The RM378 enzyme's function suggests that RM378, like T4, faces similar anti-phage mechanisms when infecting its host, Rhodothermus marinus.